Photoelectrocatalytic determination of NADH in a flow injection system with electropolymerized methylene blue

被引：37

作者：

Dilgin, Yusuf ^{[1
]}

Dilgin, Didem Giray ^{[2
,3
]}

Dursun, Zekerya ^{[3
]}

Gokcel, H. Ismet ^{[3
]}

Gligor, Delia ^{[4
]}

Bayrak, Burcu ^{[1
]}

Ertek, Bensu ^{[1
]}

机构：

[1] Canakkale Onsekiz Mart Univ, Sci & Art Fac, Dept Chem, TR-17100 Canakkale, Turkey

[2] Canakkale Onsekiz Mart Univ, Biga Vocat Coll, TR-17200 Biga Canakkale, Turkey

[3] Ege Univ, Fac Sci, Dept Chem, TR-35100 Bornova, Turkey

[4] Univ Babes Bolyai, Dept Environm Phys Chem & Engn, Cluj Napoca 400294, Romania

来源：

ELECTROCHIMICA ACTA | 2011年 / 56卷 / 03期

关键词：

Photoelectrocatalysis; Flow injection analysis; Modified electrode; NADH; Methylene blue; ELECTROCATALYTIC OXIDATION; ELECTROCHEMICAL POLYMERIZATION; ZIRCONIUM-PHOSPHATE; CARBON NANOTUBES; ASCORBIC-ACID; ELECTRODE; BIOSENSORS; DERIVATIVES; FILM;

D O I：

10.1016/j.electacta.2010.10.084

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

It was firstly described that a glassy carbon electrode electropolymerized with methylene blue shows an efficient photoelectrocatalytic activity towards NADH oxidation in a phosphate buffer solution (pH 7.0). In order to perform the photoelectrocatalytic determination of NADH in a flow injection analysis (FIA) system, a home-made flow electrochemical cell with a suitable transparent window for the irradiation of the electrode surface was constructed. The currents obtained from the photoamperometric measurements in the FIA system at optimum conditions (flow rate of carrier solution, 1.3 mL min(-1): transmission tubing length, 10 cm; injection volume, 100 mu L; and constant applied potential, +150 mV vs. Ag/AgCl) were linearly dependent on the NADH concentration and linear calibration curves were obtained in the range of 1.0 x 10(-7)-2.0 x 10(-4) M. The detection limit was found to be 4.0 x 10(-8) M for photoamperometric determination of NADH. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1138 / 1143

页数：6

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