Human growth hormone production by high cell density fermentation of recombinant Escherichia coli

被引：8

作者：

Zhang, XW ^{[1
]}

Sun, T ^{[1
]}

Liu, X ^{[1
]}

Gu, DX ^{[1
]}

Huang, XN ^{[1
]}

机构：

[1] Zhongshan Univ, State Educ Commiss, Food Engn Res Ctr, Guangzhou 510275, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 06期

关键词：

human growth hormone; high cell density fermentation; Escherichia coli; glycerol;

D O I：

10.1016/S0032-9592(98)00033-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High cell density fermentation production of recombinant human growth hormone was studied in Escherichia coli. The results showed that by using glycerol instead of glucose as carbon source and feeding in a specific fed-batch mode,by controlling the stirrer speed and keeping the dissolved oxygen level at 20-25% saturation,cell density increases from 38.6 to 118.8 g/l,the production of r-hGH remained high and the fermentation time was reduced from 16 to 10 h. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：683 / 686

页数：4

共 9 条

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JENSEN, EB
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