A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

被引：23

作者：

Iturbe-Ormaetxe, Inaki ^{[1
]}

Woolfit, Megan ^{[1
]}

Rances, Edwige ^{[1
]}

Duplouy, Anne ^{[1
]}

O'Neill, Scott L. ^{[1
]}

机构：

[1] Univ Queensland, Sch Biol Sci, Brisbane, Qld 4072, Australia

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 84卷 / 01期

基金：

澳大利亚研究理事会; 美国国家卫生研究院;

关键词：

Wolbachia pipientis; Purification; Genomics; Symbiosis; LIFE-SHORTENING WOLBACHIA; DROSOPHILA-MELANOGASTER; AEDES-AEGYPTI; STRAIN; INFECTION; PIPIENTIS; EVOLUTION; SYMBIONT; PARASITE;

D O I：

10.1016/j.mimet.2010.10.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：134 / 136

页数：3

共 23 条

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