Isolation and molecular identification of Mycobacterium bovis in tissue lesions of cattle slaughtered in slaughterhouses located in the State of Ceara

被引：1

作者：

Amorim, J. Q. ^{[1
]}

Santos, B. R. ^{[1
]}

Fehlberg, H. F. ^{[1
]}

Silva, A., V ^{[1
]}

Ferreira, F. F. ^{[2
]}

Costa, J. N. ^{[3
]}

Alzamora Filho, F. ^{[1
]}

机构：

[1] Univ Estadual Santa Cruz, Campus Soane Nazare Andrade, Ilheus, BA, Brazil

[2] Agencia Def Agr Estado Ceara, Fortaleza, Ceara, Brazil

[3] Univ Fed Reconcavo Baiano, Cruz Das Almas, BA, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2022年 / 74卷 / 02期

关键词：

diagnosis; multiplex PCR; Mycobacterium ssp; epidemiology; TUBERCULOSIS; CARCASSES; ABATTOIR; MEDIA; BREED;

D O I：

10.1590/1678-4162-12394

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective of this research was to identify Mycobacterium bovis in lesions suggestive of tuberculosis in bovine carcasses in the State of Ceara, by means of bacteriological and molecular diagnostic tests. Between August 2017 and January 2019, the State inspection service (SIE) inspected 59,512 cattle, of which 7.4% (44 / 59,512) presented suggestive lesions. Of these animals, 68 samples were sent, of which 4.5% (31/68) located in the lung, 2.9% (20/68) in lymph nodes, 2.0% (14/68) in the liver, and 0.4% in the carcass (3/68). When performing bacteriological isolation, 15.9% (7/44) of bovines showed colony growth in the samples. The smears of the isolates were submitted to Zielh-Neelsen staining and all confirmed acid-fast bacilli. The polymerase chain reaction identified all isolates 100% (7/7) as M. bovis. The association of diagnostic techniques allowed to identify the presence of the agent in the State and the molecular analysis proved to be a beneficial technique in the monitoring of bovine tuberculosis and can be used as an auxiliary method in the bovine tuberculosis control and eradication program in the State of Ceara.

引用

页码：338 / 344

页数：7

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