ChemMatrix® for complex peptides and combinatorial chemistry

被引：51

作者：

Garcia-Ramos, Yesica ^{[1
,2
]}

Paradis-Bas, Marta ^{[1
,2
]}

Tulla-Puche, Judit ^{[1
,2
]}

Albericio, Fernando ^{[1
,2
,3
]}

机构：

[1] Inst Res Biomed, Barcelona 08028, Spain

[2] Networking Ctr Bioengn Biomat & Nanomed, CIBER BBN, Barcelona 08028, Spain

[3] Univ Barcelona, Dept Organ Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF PEPTIDE SCIENCE | 2010年 / 16卷 / 12期

关键词：

solid-phase peptide synthesis; resin; PEG; combinatorial chemistry; SOLID-PHASE SYNTHESIS; PEG-BASED RESINS; POLYSTYRENE RESINS; CHEMICAL-SYNTHESIS; SUPPORTS; MICROWAVE; DIFFICULT; LIBRARIES;

D O I：

10.1002/psc.1282

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CM resin is a totally PEG-based resin, made exclusively from primary ether bonds and therefore highly chemically stable. Compared to other PEG resins, it exhibits good loading and is user friendly because of its free-flowing form upon drying. It shows improved performance over PS resins for the preparation of hydrophobic, highly structured poly-Arg peptides. In combination with psi Pros, it allows the synthesis of small proteins such as the chemokine RANTES. Like other PEG-based resins, CM resin swells well in biocompatible solvents such as water, thereby allowing on-bead screening. Furthermore, the high loading of this resin permits he use of a tiny quarter of a bead as a microreactor for HPLC and MALDI-TOF analysis, thus further extending its applications in the field of combinatorial chemistry. Copyright (C) 2010 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：675 / 678

页数：4

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