One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

被引:53
|
作者
Aveyard, Jenny
Mehrabi, Maryam
Cossins, Andrew
Braven, Helen
Wilson, Robert [1 ]
机构
[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
[2] Univ Liverpool, Dept Biol Sci, Liverpool L69 7ZD, Merseyside, England
[3] BBInternational, Cardiff CF14 5DX, Wales
关键词
D O I
10.1039/b708859k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
引用
收藏
页码:4251 / 4253
页数:3
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