One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

被引：53
作者：
Aveyard, Jenny
Mehrabi, Maryam
Cossins, Andrew
Braven, Helen
Wilson, Robert [1 ]
机构：
[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
[2] Univ Liverpool, Dept Biol Sci, Liverpool L69 7ZD, Merseyside, England
[3] BBInternational, Cardiff CF14 5DX, Wales
来源：
CHEMICAL COMMUNICATIONS | 2007年 / 41期
关键词：
D O I：
10.1039/b708859k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
引用
收藏
页码：4251 / 4253
页数：3
相关论文
共 10 条
  • [1] COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX
    BROCKLEHURST, K
    CARLSSON, J
    KIERSTAN, MP
    CROOK, EM
    [J]. BIOCHEMICAL JOURNAL, 1973, 133 (03) : 573 - 584
  • [2] Development of a quantitative lateral-flow assay for rapid detection of fatty acid-binding protein
    Chan, CPY
    Sum, KW
    Cheung, KY
    Glatz, JFC
    Sanderson, JE
    Hempel, A
    Lehmann, M
    Renneberg, I
    Renneberg, R
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 279 (1-2) : 91 - 100
  • [3] Chandler J., 2000, IVD TECHNOLOGY, V6, P37
  • [4] Gold and silver nanoparticles functionalized with known numbers of oligonucleotides per particle for DNA detection
    Chen, Y
    Aveyard, J
    Wilson, R
    [J]. CHEMICAL COMMUNICATIONS, 2004, (24) : 2804 - 2805
  • [5] Synthesis and optical properties of silver nanoparticles and arrays
    Evanoff, DD
    Chumanov, G
    [J]. CHEMPHYSCHEM, 2005, 6 (07) : 1221 - 1231
  • [6] One-step immunostrip test for the simultaneous detection of free and total prostate specific antigen in serum
    Fernández-Sánchez, C
    McNeil, CJ
    Rawson, K
    Nilsson, O
    Leung, HY
    Gnanapragasam, V
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2005, 307 (1-2) : 1 - 12
  • [7] Detection of single nucleotide substitution by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip
    Matsubara, Y
    Kure, S
    [J]. HUMAN MUTATION, 2003, 22 (02) : 166 - 172
  • [8] New rapid detection test with a combination of polymerase chain reaction and immunochromatographic assay for Mycobacterium tuberculosis complex
    Suzuki, Takeshi
    Tanaka, Masao
    Otani, Sumire
    Matsuura, Shiro
    Sakaguchic, Yoshiaki
    Nishimura, Tomoyasu
    Ishizaka, Akitoshi
    Hasegawa, Naoki
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 56 (03) : 275 - 280
  • [9] New rapid polymerase chain reaction-immunochromatographic assay for Porphyromonas gingivalis
    Takada, K
    Sakaguchi, Y
    Oka, C
    Hirasawa, M
    [J]. JOURNAL OF PERIODONTOLOGY, 2005, 76 (04) : 508 - 512
  • [10] Development of colloidal gold-based flow-through and lateral-flow immunoassays for the rapid detection of the insecticide carbaryl
    Wang, S
    Zhang, C
    Wang, JP
    Zhang, Y
    [J]. ANALYTICA CHIMICA ACTA, 2005, 546 (02) : 161 - 166
1