One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

被引:53
|
作者
Aveyard, Jenny
Mehrabi, Maryam
Cossins, Andrew
Braven, Helen
Wilson, Robert [1 ]
机构
[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
[2] Univ Liverpool, Dept Biol Sci, Liverpool L69 7ZD, Merseyside, England
[3] BBInternational, Cardiff CF14 5DX, Wales
关键词
D O I
10.1039/b708859k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
引用
收藏
页码:4251 / 4253
页数:3
相关论文
共 50 条
  • [1] Simultaneous Detection of Nucleic Acid and Protein Using Gold Nanoparticles and Lateral Flow Device
    Xun Mao
    Anant Gurung
    Hui Xu
    Meenu Baloda
    Yuqing He
    Guodong Liu
    Analytical Sciences, 2014, 30 : 637 - 642
  • [2] Simultaneous Detection of Nucleic Acid and Protein Using Gold Nanoparticles and Lateral Flow Device
    Mao, Xun
    Gurung, Anant
    Xu, Hui
    Baloda, Meenu
    He, Yuqing
    Liu, Guodong
    ANALYTICAL SCIENCES, 2014, 30 (06) : 637 - 642
  • [3] MicroRNA detection using lateral flow nucleic acid strips with gold nanoparticles
    Hou, Shao-Yi
    Hsiao, Yi-Ling
    Lin, Ming-Shu
    Yen, Chun-Che
    Chang, Chi-Sheng
    TALANTA, 2012, 99 : 375 - 379
  • [4] Visual detection of microRNA with lateral flow nucleic acid biosensor
    Gao, Xuefei
    Xu, Hui
    Baloda, Meenu
    Gurung, Anant S.
    Xu, Li-Ping
    Wang, Tao
    Zhang, Xueji
    Liu, Guodong
    BIOSENSORS & BIOELECTRONICS, 2014, 54 : 578 - 584
  • [5] Visual detection of Zika virus by isothermal nucleic acid amplification combined with a lateral-flow device
    Lin, Xuemei
    Wu, Maoyong
    Wang, Wenbiao
    Gao, Yuhang
    Zhang, Wei
    Wu, De
    Wu, Yina
    Zhou, Xiaoming
    Li, Geng
    ANALYTICAL METHODS, 2019, 11 (13) : 1795 - 1801
  • [6] Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay
    Carlos Porras, Juan
    Bernuz, Mireia
    Marfa, Jennifer
    Pallares-Rusinol, Arnau
    Marti, Merce
    Isabel Pividori, Maria
    NANOMATERIALS, 2021, 11 (03) : 1 - 11
  • [7] A facile one-step gold nanoparticles enhancement based on sequential patterned lateral flow immunoassay device for C-reactive protein detection
    Panraksa, Yosita
    Apilux, Amara
    Jampasa, Sakda
    Puthong, Songchan
    Henry, Charles S.
    Rengpipat, Sirirat
    Chailapakul, Orawon
    SENSORS AND ACTUATORS B-CHEMICAL, 2021, 329
  • [8] Femtomolar detection of nucleic acid based on functionalized gold nanoparticles
    Huang, Jiaoqi
    Zhang, Yang
    Lin, Zhongquan
    Liu, Wei
    Chen, Xueping
    Liu, Yu
    Tian, Huiyan
    Liu, Qiqian
    Gillibert, Raymond
    Spadavecchia, Jolanda
    Djaker, Nadia
    de la Chapelle, Marc Lamy
    Xiang, Yang
    Fu, Weiling
    NANOPHOTONICS, 2019, 8 (09) : 1495 - 1503
  • [9] Visual and multiplex detection of nucleic acid sequence by multiple cross displacement amplification coupled with gold nanoparticle-based lateral flow biosensor
    Wang, Yi
    Wang, Yan
    Zhang, Lu
    Xu, Jianguo
    Ye, Changyun
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 241 : 1283 - 1293
  • [10] Rapid developments in lateral flow immunoassay for nucleic acid detection
    Zheng, Chujun
    Wang, Kan
    Zheng, Wei
    Cheng, Yuemeng
    Li, Tangan
    Cao, Bo
    Jin, Qinghui
    Cui, Daxiang
    ANALYST, 2021, 146 (05) : 1514 - 1528