One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

被引:55
作者
Aveyard, Jenny
Mehrabi, Maryam
Cossins, Andrew
Braven, Helen
Wilson, Robert [1 ]
机构
[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
[2] Univ Liverpool, Dept Biol Sci, Liverpool L69 7ZD, Merseyside, England
[3] BBInternational, Cardiff CF14 5DX, Wales
关键词
D O I
10.1039/b708859k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
引用
收藏
页码:4251 / 4253
页数:3
相关论文
共 10 条
[1]   COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX [J].
BROCKLEHURST, K ;
CARLSSON, J ;
KIERSTAN, MP ;
CROOK, EM .
BIOCHEMICAL JOURNAL, 1973, 133 (03) :573-584
[2]   Development of a quantitative lateral-flow assay for rapid detection of fatty acid-binding protein [J].
Chan, CPY ;
Sum, KW ;
Cheung, KY ;
Glatz, JFC ;
Sanderson, JE ;
Hempel, A ;
Lehmann, M ;
Renneberg, I ;
Renneberg, R .
JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 279 (1-2) :91-100
[3]  
Chandler J., 2000, IVD TECHNOLOGY, V6, P37
[4]   Gold and silver nanoparticles functionalized with known numbers of oligonucleotides per particle for DNA detection [J].
Chen, Y ;
Aveyard, J ;
Wilson, R .
CHEMICAL COMMUNICATIONS, 2004, (24) :2804-2805
[5]   Synthesis and optical properties of silver nanoparticles and arrays [J].
Evanoff, DD ;
Chumanov, G .
CHEMPHYSCHEM, 2005, 6 (07) :1221-1231
[6]   One-step immunostrip test for the simultaneous detection of free and total prostate specific antigen in serum [J].
Fernández-Sánchez, C ;
McNeil, CJ ;
Rawson, K ;
Nilsson, O ;
Leung, HY ;
Gnanapragasam, V .
JOURNAL OF IMMUNOLOGICAL METHODS, 2005, 307 (1-2) :1-12
[7]   Detection of single nucleotide substitution by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip [J].
Matsubara, Y ;
Kure, S .
HUMAN MUTATION, 2003, 22 (02) :166-172
[8]   New rapid detection test with a combination of polymerase chain reaction and immunochromatographic assay for Mycobacterium tuberculosis complex [J].
Suzuki, Takeshi ;
Tanaka, Masao ;
Otani, Sumire ;
Matsuura, Shiro ;
Sakaguchic, Yoshiaki ;
Nishimura, Tomoyasu ;
Ishizaka, Akitoshi ;
Hasegawa, Naoki .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 56 (03) :275-280
[9]   New rapid polymerase chain reaction-immunochromatographic assay for Porphyromonas gingivalis [J].
Takada, K ;
Sakaguchi, Y ;
Oka, C ;
Hirasawa, M .
JOURNAL OF PERIODONTOLOGY, 2005, 76 (04) :508-512
[10]   Development of colloidal gold-based flow-through and lateral-flow immunoassays for the rapid detection of the insecticide carbaryl [J].
Wang, S ;
Zhang, C ;
Wang, JP ;
Zhang, Y .
ANALYTICA CHIMICA ACTA, 2005, 546 (02) :161-166