Molecular engineering of chromophores for combined second-harmonic and two-photon fluorescence in cellular imaging

被引：56

作者：

De Meulenaere, Evelien ^{[2
,4
,5
]}

Chen, Wei-Qiang ^{[1
,3
]}

Van Cleuvenbergen, Stijn ^{[2
]}

Zheng, Mei-Ling ^{[1
,3
]}

Psilodimitrakopoulos, Sotiris ^{[6
]}

Paesen, Rik ^{[7
]}

Taymans, Jean-Marc ^{[8
]}

Ameloot, Marcel ^{[7
]}

Vanderleyden, Jos ^{[4
,5
]}

Loza-Alvarez, Pablo ^{[6
]}

Duan, Xuan-Ming ^{[1
,3
]}

Clays, Koen ^{[2
,5
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Organ NanoPhoton, Beijing 100190, Peoples R China

[2] Univ Louvain, Dept Chem, B-3001 Louvain, Belgium

[3] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Funct Crystals & Laser Technol, Beijing 100190, Peoples R China

[4] Univ Louvain, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium

[5] Univ Louvain, Inst Nanoscale Phys & Chem INPAC, B-3001 Louvain, Belgium

[6] Inst Photon Sci, ICFO, Castelldefels 08860, Barcelona, Spain

[7] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium

[8] Univ Louvain, Lab Neurobiol & Gene Therapy, B-3001 Louvain, Belgium

来源：

CHEMICAL SCIENCE | 2012年 / 3卷 / 04期

关键词：

HUMAN TELOMERES; GENERATION; MICROSCOPY; SHG; 2ND; HYPERPOLARIZABILITIES; CELLS;

D O I：

10.1039/c2sc00771a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A series of chromophores with enhanced second-and third-order nonlinear optical properties were engineered for use in combined second-harmonic and two-photon fluorescence microscopy. Electronaccepting moieties imparted nonlinear optical properties to the chromophores. The electron-rich carbazole core served as a template towards one-or two-dimensional chromophores. More efficient acceptor groups (pyridinium, benzazolium, benzothiazolium) on the carbazole donor core resulted in improved second-and third-order nonlinear optical properties. A selection of these chromophores was tested in a cellular environment with a multimodal multiphoton microscope. The structural differences of the chromophores resulted in high selectivity for mitochondria or the nucleus in two-photon fluorescence and ranging from no signal to high selectivity for mitochondria in the SHG channel.

引用

页码：984 / 995

页数：12

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