A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo

被引：48

作者：

Li, Yuyao ^{[1
]}

Song, Heng ^{[1
]}

Xue, Chenghong ^{[1
]}

Fang, Zhijun ^{[1
]}

Xiong, Liqin ^{[2
]}

Xie, Hexin ^{[1
]}

机构：

[1] East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai Med X Engn Ctr Med Equipment & Technol, Shanghai 200030, Peoples R China

来源：

CHEMICAL SCIENCE | 2020年 / 11卷 / 23期

关键词：

LACZ-POSITIVE CELLS; FLUORESCENT-PROBES; ALKALINE-PHOSPHATASE; MECHANISM;

D O I：

10.1039/d0sc01273d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system.

引用

收藏

页码：5889 / 5894

页数：6

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