Molecular investigation of transplacental and vector-borne transmission of bovine haemoplasmas

被引：61
作者：
Hornok, S. [1 ]
Micsutka, A. [1 ]
Meli, M. L. [2 ]
Lutz, H. [2 ]
Hofmann-Lehmann, R. [2 ]
机构：
[1] Szent Istvan Univ, Fac Vet Sci, Dept Parasitol & Zool, H-1078 Budapest, Hungary
[2] Univ Zurich, Vetsuisse Fac, Clin Lab, CH-8057 Zurich, Switzerland
来源：
VETERINARY MICROBIOLOGY | 2011年 / 152卷 / 3-4期
关键词：
Mycoplasma; Haemoplasma; Transplacental; Vector; Diptera; CANDIDATUS MYCOPLASMA HAEMOBOS; EPERYTHROZOON-WENYONII; INFECTION; CATTLE;
D O I：
10.1016/j.vetmic.2011.04.031
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The present study was carried out in a herd with concurrent infections of Mycoplasma wenyonii and 'Candidatus M. haemobos', to investigate if transplacental and/or vector-borne transmission is possible for one or both bovine haemoplasma species. For this purpose blood samples were collected from 38 mother animals and their newborn calves; as well as from 17 uninseminated cows twice three months apart. In addition, 311 mosquitoes and blood-sucking flies (Diptera: Culicidae, Tabanidae. Muscidae) were cought near the animals. DNA was extracted from all samples, followed by real-time PCR analysis. In 10.5% of neonate calves, that were born to cows harbouring both haemoplasmas, M. wenyonii and/or 'Candidatus M. haemobos' positivity was detected. Copy numbers in positive samples from cows and their calves indicated that - in comparison with M. wenyonii - 'Candidatus M. haemobos'-bacteraemia had usually lower levels. In samples of uninseminated cows the rate of infection with the latter species decreased. These findings may explain why M. wenyonii was significantly more frequently detected in blood-sucking flies, than 'Candidatus M. haemobos'. In conclusion, molecular evidence is provided for the first time on the transplacental transmission of bovine haemoplasmas. Regarding their spread by blood-sucking arthropods, new potential vectors were identified, i.e. the horn fly (Haematobia irritans), the stable fly (Stomoxys calcitrans) and two species of horse flies (Tabanus bovinus, T. bromius). (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：411 / 414
页数：4
相关论文
共 21 条
[1]  
Almy FS, 2006, CAN VET J, V47, P229
[2]  
BERRIER HH, 1954, J AM VET MED ASSOC, V124, P98
[3]   Molecular Investigations of Rickettsia helvetica Infection in Dogs, Foxes, Humans, and Ixodes Ticks [J].
Boretti, Felicitas S. ;
Perreten, Andrea ;
Meli, Marina L. ;
Cattori, Valentino ;
Willi, Barbara ;
Wengi, Nicole ;
Hornok, Sandor ;
Honegger, Hanspeter ;
Hegglin, Daniel ;
Woelfel, Roman ;
Reusch, Claudia E. ;
Lutz, Hans ;
Hofmann-Lehmann, Regina .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (10) :3230-3237
[4]   CULEX-ANNULIROSTRIS AS A VECTOR OF EPERYTHROZOON-OVIS INFECTION IN SHEEP [J].
DADDOW, KN .
VETERINARY PARASITOLOGY, 1980, 7 (04) :313-317
[5]   TABANIDS AS VECTORS OF DISEASE AGENTS [J].
FOIL, LD .
PARASITOLOGY TODAY, 1989, 5 (03) :88-96
[6]   'Candidatus Mycoplasma haemobos', a new bovine haemotrophic Myco plasma species? [J].
Hoelzle, Katharina ;
Hofmann-Lehmann, Regina ;
Hoelzle, Ludwig E. .
VETERINARY MICROBIOLOGY, 2010, 144 (3-4) :525-526
[7]   Detection of Candidatus Mycoplasma haemobos in cattle with anaemia [J].
Hoelzle, Katharina ;
Winkler, Margit ;
Kramer, Manuela M. ;
Wittenbrink, Max M. ;
Dieckmann, Sarah M. ;
Hoelzle, Ludwig E. .
VETERINARY JOURNAL, 2011, 187 (03) :408-410
[8]   Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland [J].
Hofmann-Lehmann, R ;
Meli, ML ;
Dreher, UM ;
Gönczi, E ;
Deplazes, P ;
Braun, U ;
Engels, M ;
Schüpbach, J ;
Jörger, K ;
Thoma, R ;
Griot, C ;
Stärk, KDC ;
Willi, B ;
Schmidt, J ;
Kocan, KM ;
Lutz, H .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3775-3780
[9]   Fatal bovine anaplasmosis in a herd with new genotypes of Anaplasma marginale, Anaplasma ovis and concurrent haemoplasmosis [J].
Hornok, S. ;
Micsutka, A. ;
Fernandez de Mera, I. G. ;
Meli, M. L. ;
Goenczi, E. ;
Tanczos, B. ;
Mangold, A. J. ;
Farkas, R. ;
Lutz, H. ;
Hofmann-Lehmann, R. ;
de la Fuente, J. .
RESEARCH IN VETERINARY SCIENCE, 2012, 92 (01) :30-35
[10]   Survey on blood-sucking lice (Phthiraptera: Anoplura) of ruminants and pigs with molecular detection of Anaplasma and Rickettsia spp [J].
Hornok, S. ;
Hofmann-Lehmann, R. ;
Fernandez de Mera, I. G. ;
Meli, M. L. ;
Elek, V. ;
Hajtos, I. ;
Repasi, A. ;
Goenczi, E. ;
Tanczos, B. ;
Farkas, R. ;
Lutz, H. ;
de la Fuente, J. .
VETERINARY PARASITOLOGY, 2010, 174 (3-4) :355-358
123