Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality

被引：63

作者：

Cariati, Federica ^{[1
,2
]}

Jaroudi, Souraya ^{[1
]}

Alfarawati, Samer ^{[1
]}

Raberi, Araz ^{[1
]}

Alviggi, Carlo ^{[3
]}

Pivonello, Rosario ^{[4
]}

Wells, Dagan ^{[1
,5
]}

机构：

[1] Inst Reprod Sci, Reprogenet UK, Oxford Business Pk North, Oxford OX4 2HW, England

[2] CEINGE Biotecnol Avanzate Scarl, Via Gaetano Salvatore 486, I-80145 Naples, Italy

[3] Univ Naples Federico II, Dept Neurosci, Reprod Med, Odontostomatol, Via S Pansini 5, I-80131 Naples, Italy

[4] Univ Naples Federico II, Endocrinol Sect, Dept Med & Surg, Via S Pansini 5, I-80131 Naples, Italy

[5] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Obstet & Gynaecol, Oxford OX3 9DU, England

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2016年 / 33卷 / 03期

关键词：

aneuploidy; human sperm; male infertility; sperm DNA damage; telomere length; IN-SITU HYBRIDIZATION; CHROMOSOME ANEUPLOIDY; LONGER TELOMERES; DNA; SPERMATOZOA; MEN; AGE; CELLS; RATES; ULTRASTRUCTURE;

D O I：

10.1016/j.rbmo.2016.06.006

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Recent studies have reported shorter sperm telomere length (STL) in men with idiopathic infertility. The aim of this study was to measure STL in semen samples from men to evaluate whether STL variation is associated with chromosomal abnormality, DNA fragmentation, traditional semen parameters, IVF outcome, or all four factors. A significant correlation between telomere length and diploidy was observed (P = 0.037). Additionally, STL was found to be positively associated with sperm count (P = 0.006); oligospermic samples had particularly short telomeres (0.9 +/- 0.1 versus 1.4 +/- 0.1; P = 0.0019). The results confirmed a link between sperm DNA fragmentation and aneuploidy, previously proposed (P = 0.009). A negative relationship was demonstrated between sperm concentration and aneuploidy and Sperm DNA framentation (P = 0.03, P < 0.0001, respectively). For a subset of 51 of the 73 sperm samples used for fertilization, IVF outcomes were known. A total of 17.6% of these samples had atypical STLs. None of these samples produced an ongoing pregnancy. In contrast, the pregnancy rate for samples that had STLs in the normal range was 35.7% (P = 0.044). In conclusion, STL has potential as a fast and inexpensive form of sperm quality assessment. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

引用

页码：404 / 411

页数：8

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