Effect of Ag-1031 on apoptosis in gastric cancer AGS cells and its effects on the PI3K/AKT/mTOR signaling pathway

被引：13
作者：
Dai, Jianhua [1 ]
Liu, Danfeng [1 ]
Chen, Longjiang [1 ]
Sun, LiXia [1 ]
机构：
[1] Second Peoples Hosp Wuhu, Dept Hepatobiliary Surg, 259 Jiuhua Middle Rd, Wuhu 241001, Anhui, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2020年 / 42卷 / 11期
关键词：
Ag-1031; Gastric cancer; Apoptosis; PI3K; AKT; mTOR signaling pathway; CYCLE ARREST; 4; PC4; AUTOPHAGY;
D O I：
10.1007/s10529-020-02954-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Purpose To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway. Methods Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method. Results Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR. Conclusion In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.
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收藏
页码：2447 / 2452
页数：6
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