A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry

被引：40

作者：

Kuyama, Hiroki ^{[1
]}

Shima, Keisuke ^{[2
]}

Sonomura, Kazuhiro ^{[2
]}

Yamaguchi, Minoru ^{[2
]}

Ando, Eiji ^{[2
]}

Nishimura, Osamu ^{[1
,2
]}

Sunasawa, Susumu ^{[1
,2
]}

机构：

[1] Osaka Univ, Inst Protein Res, Suita, Osaka 5650871, Japan

[2] Shimadzu Co Ltd, Life Sci Lab, Kyoto, Japan

来源：

PROTEOMICS | 2008年 / 8卷 / 08期

关键词：

C-terminal peptide; isolation; mass spectrometry; post-translational modification; sequencing;

D O I：

10.1002/pmic.200701044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and efficient method for C-terminal sequencing of proteins has long been pursued because it would provide substantial information for identifying the covalent structure, including post-translational modifications. However, there are still significant impediments to both direct sequencing from C termini of proteins and specific isolation of C-terminal peptides from proteins. We describe here a highly successful, de novo C-terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6-trimethoxyphenyl) phosphonium bromide and mass spectrometry.

引用

页码：1539 / 1550

页数：12

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