Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid

被引：117

作者：

Kugimiya, A ^{[1
]}

Takeuchi, T ^{[1
]}

机构：

[1] Hiroshima City Univ, Fac Informat Sci, Lab Synthet Biochem, Asaminami Ku, Hiroshima 7313194, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期

关键词：

surface plasmon resonance; molecular imprinting; sialic acid; ganglioside; biomimetic sensor;

D O I：

10.1016/S0956-5663(01)00227-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：1059 / 1062

页数：4

共 16 条

[1] Surface plasmon resonance imaging measurements of DNA hybridization adsorption and streptavidin/DNA multilayer formation at chemically modified gold surfaces [J].