Formation of droplet interface bilayers in a Teflon tube

被引:7
作者
Walsh, Edmond [1 ]
Feuerborn, Alexander [2 ]
Cook, Peter R. [2 ]
机构
[1] Univ Oxford, Dept Engn Sci, Osney Thermofluids Lab, Southwell Bldg, Oxford OX2 0ES, England
[2] Univ Oxford, Sir William Dunn Sch Pathol, South Parks Rd, Oxford OX1 3RE, England
来源
SCIENTIFIC REPORTS | 2016年 / 6卷
基金
英国医学研究理事会;
关键词
TECHNOLOGIES; MONOLAYERS; MEMBRANES; ARRAY;
D O I
10.1038/srep34355
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Droplet-interface bilayers (DIBs) have applications in disciplines ranging from biology to computing. We present a method for forming them manually using a Teflon tube attached to a syringe pump; this method is simple enough it should be accessible to those without expertise in microfluidics. It exploits the properties of interfaces between three immiscible liquids, and uses fluid flow through the tube to pack together drops coated with lipid monolayers to create bilayers at points of contact. It is used to create functional nanopores in DIBs composed of phosphocholine using the protein alpha-hemolysin (alpha HL), to demonstrate osmotically-driven mass transfer of fluid across surfactant-based DIBs, and to create arrays of DIBs. The approach is scalable, and thousands of DIBs can be prepared using a robot in one hour; therefore, it is feasible to use it for high throughput applications.
引用
收藏
页数:9
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