Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

被引：13

作者：

Ngo, Jennifer ^{[1
,2
,3
]}

Benador, Ilan Y. ^{[1
]}

Brownstein, Alexandra J. ^{[1
,3
]}

Vergnes, Laurent ^{[3
,4
]}

Veliova, Michaela ^{[1
,3
]}

Shum, Michael ^{[1
,3
]}

Acin-Perez, Rebeca ^{[1
,3
]}

Reue, Karen ^{[3
,4
,5
]}

Shirihai, Orian S. ^{[1
,3
]}

Liesa, Marc ^{[1
,3
,5
]}

机构：

[1] David Geffen Sch Med UCLA, Dept Med, Div Endocrinol, Los Angeles, CA 90095 USA

[2] UCLA, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[3] UCLA, Metab Theme, David Geffen Sch Med, Los Angeles, CA 90095 USA

[4] UCLA, Dept Human Genet, David Geffen Sch Med, Los Angeles, CA 90024 USA

[5] UCLA, Mol Biol Inst, Los Angeles, CA 90095 USA

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 01期

关键词：

Metabolism; Molecular biology;

D O I：

10.1016/j.xpro.2020.100243

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondria play a central role in lipid metabolism and can bind to lipid drop-lets. However, the role and functional specialization of the population of peri-droplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry.For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

引用

页数：19

共 50 条

[1] Recruitment and remodeling of peridroplet mitochondria in human adipose tissue
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