Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor

被引：7
|
作者：
Ji, Xue Ping [1 ]
Li, Xian Rui [1 ]
Wang, Na [1 ]
Ni, Rui Xing [1 ]
Liu, Xiao Hong [1 ]
Xiong, Hua Ai [1 ]
机构：
[1] Hebei Med Univ, Dept Med Chem, Shijiazhuang 050017, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2010年 / 21卷 / 10期
关键词：
Biosensor; Tyrosinase; Catechol; Self-assembled monolayers; Gold electrode; DISK ENZYME ELECTRODE; PHENOLIC-COMPOUNDS; GLUCOSE-OXIDASE;
D O I：
10.1016/j.cclet.2010.05.022
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A mixed self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic acid amide (T-NH2) were used to immobilize tyrosinase for fabricating biosensor. The results showed that the mixed SAMs prepared from solution at the ratio of 1:4 provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate. The biosensor exhibited a fast response and high sensitivity for sensing substrate. (C) 2010 Xue Ping Ji. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
引用
收藏
页码：1239 / 1242
页数：4
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