Intramolecular cross-linking of proteins with azobenzene-based cross-linkers

被引：5

作者：

Yasuike, Nobuo ^{[1
,2
]}

Lu, Huixin ^{[1
]}

Xia, Peng ^{[1
]}

Woolley, G. Andrew ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON, Canada

[2] JSR Corp, Tokyo, Japan

来源：

OPTOCHEMICAL BIOLOGY | 2019年 / 624卷

关键词：

PHOTO-CONTROL; PHOTOSWITCHES; RED; TRIS(2-CARBOXYETHYL)PHOSPHINE; DESIGN; LIGHT;

D O I：

10.1016/bs.mie.2019.04.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The photo-control of protein activity can often be achieved via the photo-control of protein structure. Intramolecular cross-linkers that change length upon photoisomerization provide a means to photo-control protein structure by linking to pairs of Cys residues in a protein sequence. In this protocol, we describe general methods for introducing intramolecular cross-linkers, both UV light switchable and red-light switchable, under either denaturing or native conditions.

引用

页码：129 / 149

页数：21

共 50 条

[1] Cross-linking Copolymerization of Acrylic Acid and Multi-armed Cross-linkers
Qiang CHEN1
Chinese Chemical Letters, 2001, (11) : 1029 - 1032
[2] NEW THERMAL CROSS-LINKERS BASED ON TRIAZENE - CROSS-LINKING OF FLUORINATED POLYIMIDES AND AROMATIC POLYMERS
LAU, ANK
VO, LP
MACROMOLECULES, 1992, 25 (26) : 7294 - 7299
[3] Cross-linking copolymerization of acrylic acid and multi-armed cross-linkers
Chen, Q
Guan, Y
Zhang, XM
Peng, YX
Xu, J
CHINESE CHEMICAL LETTERS, 2001, 12 (11) : 1029 - 1032
[4] Quantitative cross-linking/mass spectrometry using isotope-labelled cross-linkers
Fischer, Lutz
Chen, Zhuo Angel
Rappsilber, Jun
JOURNAL OF PROTEOMICS, 2013, 88 : 120 - 128
[5] Cross-Linking Vitrimers after Melt Processing Using Supramolecularly Masked Dynamic Cross-Linkers
Formon, Georges J. M.
Jayaratnam, Jonathan
Guibert, Clement
Van Zee, Nathan J.
Nicolay, Renaud
MACROMOLECULES, 2024, 57 (17) : 8277 - 8286
[6] Quantitative Cross-linking/Mass Spectrometry Using Isotope-labeled Cross-linkers and MaxQuant
Chen, Zhuo A.
Fischer, Lutz
Cox, Juergen
Rappsilber, Juri
MOLECULAR & CELLULAR PROTEOMICS, 2016, 15 (08) : 2769 - 2778
[7] The Conformational Preference of Chemical Cross-linkers Determines the Cross-linking Probability of Reactive Protein Residues
Gong, Zhou
Ye, Shang-Xiang
Nie, Ze-Feng
Tang, Chun
JOURNAL OF PHYSICAL CHEMISTRY B, 2020, 124 (22): : 4446 - 4453
[8] Recombinant proteins as cross-linkers for hydrogelations
Wang, Huaimin
Shi, Yang
Wang, Ling
Yang, Zhimou
CHEMICAL SOCIETY REVIEWS, 2013, 42 (03) : 891 - 901
[9] Changes in Heat Resistance and Mechanical Properties of Peroxide Cross-Linking HDPE: Effects of Compounding Cross-Linkers
Liu, Shunquan
Zhang, Run
Fu, Chenchao
Zheng, Tianshuo
Xue, Ping
POLYMERS, 2025, 17 (04)
[10] Tuning the Permeability of Polymer Hydrogel Capsules: An Investigation of Cross-Linking Density, Membrane Thickness, and Cross-Linkers
Chong, Siow-Feng
Lee, Ji Hyun
Zelikin, Alexander N.
Caruso, Frank
LANGMUIR, 2011, 27 (05) : 1724 - 1730

← 1 2 3 4 5 →