Global Genomic Characterization of Salmonella enterica Serovar Telelkebir

被引:25
作者
Qiu, Yu-feng [1 ,2 ]
Nambiar, Reshma B. [3 ,4 ]
Xu, Xue-bin [5 ]
Weng, Shun-tai [1 ,2 ]
Pan, Hang [3 ,4 ]
Zheng, Kui-cheng [1 ,2 ,6 ]
Yue, Min [3 ,4 ,7 ,8 ,9 ]
机构
[1] Fujian Prov Ctr Dis Control & Prevent, Dept Bacterial, Fuzhou, Peoples R China
[2] Dept Bacterial, Fujian Prov Key Lab Zoonosis Res, Fuzhou, Peoples R China
[3] Zhejiang Univ, Dept Vet Med, Coll Anim Sci, Hangzhou, Peoples R China
[4] Zhejiang Univ, Inst Prevent Vet Sci, Coll Anim Sci, Hangzhou, Peoples R China
[5] Shanghai Municipal Ctr Dis Control & Prevent, Dept Microbiol, Shanghai, Peoples R China
[6] Fujian Med Univ, Sch Publ Hlth, Fuzhou, Peoples R China
[7] Zhejiang Prov Key Lab Prevent Vet Med, Hangzhou, Peoples R China
[8] Zhejiang Univ, State Key Lab Diag & Treatment Infect Dis, Natl Clin Res Ctr Infect Dis, Natl Med Ctr Infect Dis,Coll Med,Affiliated Hosp, Hangzhou, Peoples R China
[9] Zhejiang Univ, Hainan Inst, Sanya, Peoples R China
关键词
Salmonella Telelkebir; invasive infection; antimicrobial resistance gene; typhoid toxin; virulence gene; MOLECULAR CHARACTERIZATION; ANTIMICROBIAL RESISTANCE; INFECTION-CONTROL; TYPHOID TOXIN; REAL-TIME; FOOD; PREVALENCE; TOOL;
D O I
10.3389/fmicb.2021.704152
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Non-typhoidal Salmonella (NTS) is a common cause for self-limiting gastroenteritis, representing a public health concern globally. NTS is one of the leading causes of foodborne illnesses in China; however, the invasive infection caused by NTS is largely underappreciated. Here, we reported an NTS invasive infection caused by an infrequently reported serovar Telelkebir (13,23:d:e,n,z15) strain FJ001 in China, which carries antimicrobial-resistant genes [fosA7 and aac(6')-Iaa] and typhoidtoxin genes (cdtB, pltA, and pltB). By conducting the whole genomic sequencing, we also investigated the relatedness of this strain with an additional 120 global contextual Salmonella enterica serovar Telelkebir (S. Telelkebir) isolates, and assessed the antimicrobial-resistant determinants and key virulence factors using the available genomic dataset. Notably, all 121 (100%) of the S. Telelkebir strains possessed the typhoid toxin genes cdtB, pltA, and pltB, and 58.67% (71/121) of S. Telelkebir harbored antimicrobial-resistant gene fosaA7. The study by core genome multilocus sequence typing (cgMLST) and core single-nucleotide polymorphism (SNP)-based phylogenomic analysis demonstrated that the S. Telelkebir isolates from different sources and locations clustered together. This suggests that regular international travels might increase the likelihood of rapid and extensive transmissions of potentially pathogenic bacteria. For the first time, our study revealed the antimicrobial resistance, virulence patterns, and genetic diversity of the serovar S. Telelkebir isolate in humans and similar isolates over the world. The present study also suggests that genomic investigation can facilitate surveillance and could offer added knowledge of a previously unknown threat with the unique combination of virulent and antimicrobial-resistant determinants.
引用
收藏
页数:11
相关论文
共 64 条
[1]   Mechanisms of typhoid toxin neutralization by antibodies targeting glycan receptor binding and nuclease subunits [J].
Ahn, Changhwan ;
Yang, Yi-An ;
Neupane, Durga P. ;
Nguyen, Tri ;
Richards, Angelene F. ;
Sim, Ji Hyun ;
Mantis, Nicholas J. ;
Song, Jeongmin .
ISCIENCE, 2021, 24 (05)
[2]   Antimicrobial resistance and virulence factors profile of Salmonella spp. and Escherichia coli isolated from different environments exposed to anthropogenic activity [J].
Balbin, Michelle M. ;
Hull, Dawn ;
Guest, Chloe ;
Nichols, Lauren ;
Dunn, Robert ;
Thakur, Siddhartha .
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 22 :578-583
[3]   Splenic abscesses caused by a reptile-associated salmonella infection [J].
Berendes, T. D. ;
Keijman, J. M. G. ;
Velde, L. F. te ;
Oostenbroek, R. J. .
DIGESTIVE SURGERY, 2007, 24 (05) :397-399
[4]   Emergence and Dissemination of mcr-Carrying Clinically Relevant Salmonella Typhimurium Monophasic Clone ST34 [J].
Biswas, Silpak ;
Li, Yan ;
Elbediwi, Mohammed ;
Yue, Min .
MICROORGANISMS, 2019, 7 (09)
[5]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[6]   β-Lactams and β-Lactamase Inhibitors: An Overview [J].
Bush, Karen ;
Bradford, Patricia A. .
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2016, 6 (08)
[7]   Pan-genomic analysis of the species Salmonella enterica: Identification of core essential and putative essential genes [J].
Chand, Yamini ;
Alam, Md. Afroz ;
Singh, Sachidanand .
GENE REPORTS, 2020, 20
[8]   Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen [J].
Chang, Shu-Jung ;
Jin, Sheng Chih ;
Jiao, Xuyao ;
Galan, Jorge E. .
PLOS PATHOGENS, 2019, 15 (04)
[9]  
Chong A, 2017, YALE J BIOL MED, V90, P283
[10]   Development and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance determinants [J].
Ciesielczuk, H. ;
Hornsey, M. ;
Choi, V. ;
Woodford, N. ;
Wareham, D. W. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 62 :1823-1827