Variation of penicillin acylase partition coefficient with phase volume ratio in poly(ethylene glycol) sodium citrate aqueous two-phase systems

被引：39
作者：
Marcos, JC
Fonseca, LP
Ramalho, MT
Cabral, JMS
机构：
[1] Univ Minho, Inst Biotecnol & Quim Fina Polo Braga, P-4710 Braga, Portugal
[2] Inst Super Tecn, Ctr Engn Biol & Quim, P-1000 Lisbon, Portugal
来源：
JOURNAL OF CHROMATOGRAPHY B | 1998年 / 711卷 / 1-2期
关键词：
aqueous two-phase systems; partitioning; penicillin acylase; enzymes;
D O I：
10.1016/S0378-4347(97)00633-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The influence of phase volume ratio on partition and purification of penicillin acylase from Escherichia coli on poly(ethylene glycol)-sodium citrate aqueous two-phase systems was studied. In PEG 1000 systems both partition coefficients of the enzyme and total protein increased with decreasing phase volume ratio. However, in PEG 3350 containing NaCl, penicillin acylase follows a reverse trend, while total protein behaves in the same way. Implications for protein purification designs are discussed. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：295 / 299
页数：5
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