In vitro evaluation of gastrointestinal survival of Lactobacillus amylovorus DSM 16698 alone and combined with galactooligosaccharides, milk and/or Bifidobacterium animalis subsp lactis Bb-12

被引:39
作者
Martinez, Rafael C. R. [2 ,3 ]
Aynaou, Azz-Eddine [4 ]
Albrecht, Simone [5 ]
Schols, Henk A. [5 ]
De Martinis, Elaine C. P. [6 ]
Zoetendal, Erwin G. [3 ]
Venema, Koen [4 ]
Saad, Susana M. I. [1 ]
Smidt, Hauke [3 ]
机构
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Tecnol Bioquim Farmaceut, BR-05508000 Sao Paulo, Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, BR-05508000 Sao Paulo, Brazil
[3] Wageningen Univ, Microbiol Lab, NL-6703 HB Wageningen, Netherlands
[4] TNO Hlth Living, NL-3700 AJ Zeist, Netherlands
[5] Wageningen Univ, Food Chem Lab, NL-6703 HD Wageningen, Netherlands
[6] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil
关键词
Lactobacillus; Probiotic; Galactooligosaccharides; In vitro survival; Bifidobacterium; Milk; BILE-SALT HYDROLASE; GALACTO-OLIGOSACCHARIDES; ACID BACTERIA; STOMACH; GROWTH; GUT; DIFFERENTIATION; ACIDOPHILUS; PREBIOTICS; MICROBIOTA;
D O I
10.1016/j.ijfoodmicro.2011.06.010
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Probiotic properties of Lactobacillus amylovorus DSM 16698 were previously demonstrated in piglets. Here, its potential as a human probiotic was studied in vitro, using the TIM-1 system, which is fully validated to simulate the human upper gastrointestinal tract. To evaluate the effect of the food matrix composition on the survival of L amylovorus DSM 16698 in TIM-1, the microorganism was inoculated alone or with prebiotic galactooligosaccharides (GOS), partially skimmed milk (PSM) and/or commercial probiotic Bifidobacterium animalis subsp. lactis Bb-12 (Bb-12). Samples were collected from TIM-1 for six hours, at one-hour intervals and L amylovorus populations were enumerated on MRS agar plates with confirmation of identity of selected isolates by randomly amplified polymorphic DNA (RAPD) fingerprinting. The cumulative survival for L amylovorus alone (control) was 30% at the end of the experiment (t = 6 h). Co-administration of L amylovorus with GOS. PSM and/or Bb-12 increased its survival in comparison with the control significantly from the 4th hour after ingestion onwards (P<0.05). Furthermore, by the use of High Performance Anion Exchange Chromatography, both L amylovorus and Bb-12 were observed to promptly degrade GOS compounds in samples collected from TIM-1, as assessed at t = 2 h. Hence, food matrix composition interfered with survival and growth of L. amylovorus during passage through TIM-1, providing leads towards optimization of probiotic properties in vivo. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:152 / 158
页数:7
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