Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture

被引：9

作者：

Sasayama, N

Kashiwakura, I

Tokushima, Y

Xu, Y

Wada, S

Murakami, M

Hayase, Y

Takagi, Y

Takahashi, TA

机构：

[1] Univ Tokyo, Inst Med Sci, Cell Proc Dept, Minato Ku, Tokyo 1080071, Japan

[2] Hokkaido Coll Pharm, Lab Radiopharmaceut Sci, Otaru, Hokkaido, Japan

来源：

CYTOTHERAPY | 2001年 / 3卷 / 02期

关键词：

D O I：

10.1080/14653240152584686

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Long-term severe thrombocytopenia following human placental and umbilical cord blood (CB) transplantation is a significant clinical problem. We studied the ex vivo expansion of megakaryocytic progenitor cells (CFU-Meg) from cryopreserved/thawed leukocyte concentrates (LC) of CB prepared by the Tokyo Cord Blood Bank protocol. The LC cells were cultured in serum-free culture medium supplemented with a combination of early-acting cytokines including thrombopoietin (TPO), flt3-ligand (FL), and stem cell factor (SCF). Combination of TPO plus FL, TPO plus SCF, and all of these cytokines together resulted in 8.9-, 7.7-, and 8.4-fold increases in CFU-Meg, respectively, by Day 5 of culture. Our results showed that this simple expansion strategy has the potential for expanding CFU-Meg from cryopreserved/thawed LC cells from CB.

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页码：117 / 126

页数：10

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