Single cell genome analysis supports a link between phagotrophy and primary plastid endosymbiosis

被引：43
作者：
Bhattacharya, Debashish [1 ,2 ]
Price, Dana C. [1 ,2 ]
Yoon, Hwan Su [3 ]
Yang, Eun Chan [4 ]
Poulton, Nicole J. [4 ]
Andersen, Robert A. [5 ]
Das, Sushma Parankush [1 ,2 ]
机构：
[1] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA
[2] Rutgers State Univ, Inst Marine & Coastal Sci, New Brunswick, NJ 08901 USA
[3] Sungkyunkwan Univ, Dept Biol Sci, Suwon 440746, South Korea
[4] Bigelow Lab Ocean Sci, W Boothbay Harbor, ME USA
[5] Univ Washington, Friday Harbor Labs, Friday Harbor, WA 98250 USA
来源：
SCIENTIFIC REPORTS | 2012年 / 2卷
基金：
美国国家科学基金会;
关键词：
HORIZONTAL GENE-TRANSFER; PHOTOSYNTHETIC EUKARYOTES; N-SP; EVOLUTION; AMPLIFICATION; ORGANELLE; ORIGIN; PROCHLOROCOCCUS; ACQUISITION; EUGLYPHINA;
D O I：
10.1038/srep00356
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Two cases of primary plastid endosymbiosis are known. The first occurred ca. 1.6 billion years ago and putatively gave rise to the canonical plastid in algae and plants. The second is restricted to a genus of rhizarian amoebae that includes Paulinella chromatophora. Photosynthetic Paulinella species gained their plastid from an alpha-cyanobacterial source and are sister to plastid-lacking phagotrophs such as Paulinella ovalis that ingest cyanobacteria. To study the role of feeding behavior in plastid origin, we analyzed single-cell genome assemblies from six P. ovalis-like cells isolated from Chesapeake Bay, USA. Dozens of contigs in these cell assemblies were derived from prey DNA of alpha-cyanobacterial origin and associated cyanophages. We found two examples of horizontal gene transfer (HGT) in P. ovalis-like nuclear DNA from cyanobacterial sources. This work suggests the first evidence of a link between feeding behavior in wild-caught cells, HGT, and plastid primary endosymbiosis in the monophyletic Paulinella lineage.
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页数：8
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