PCR-based assay for the detection of Alternaria species and correlation with HPLC determination of altenuene, alternariol and alternariol monomethyl ether production in tomato products

被引：43
作者：
Angel Pavon, Miguel [1 ]
Luna, Agustin [2 ]
de la Cruz, Silvia [1 ]
Gonzalez, Isabel [1 ]
Martin, Rosario [1 ]
Garcia, Teresa [1 ]
机构：
[1] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain
[2] Univ Nacl Cordoba, Inst Ciencia & Tecnol Alimentos, Fac Ciencias Exactas Fis & Nat, RA-5016 Cordoba, Argentina
来源：
FOOD CONTROL | 2012年 / 25卷 / 01期
关键词：
Alternaria spp; PCR; Mycotoxin; HPLC; Tomato products; REAL-TIME PCR; NATURAL OCCURRENCE; MYCOTOXINS ALTERNARIOL; LIQUID-CHROMATOGRAPHY; TENUAZONIC ACID; TOXINS; CONTAMINATION; METABOLITES; FUSARIUM; WHEAT;
D O I：
10.1016/j.foodcont.2011.10.009
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Alternaria spp. contamination and subsequent production of mycotoxins is a common problem in vegetable crops. Identification of Alternaria species by traditional methods requires specific skills and may not detect toxigenic moulds inactivated by food processing. By using molecular methods such as PCR the detection of Alternaria spp. becomes possible directly from the food or feed samples. In this study, a PCR method based on the Internal Transcribed Spacer (ITS) genetic marker has been used for detection of Alternaria spp. in raw and processed commercial tomato samples. Occurrence of altenuene, alternariol and alternariol methyl ether in the samples was analysed by high-performance liquid chromatography (HPLC) in order to assess the ability of the PCR assay to identify tomato samples containing Alternaria mycotoxins. The PCR assay revealed the presence of Alternaria spp. DNA in 41 out of 90 commercial samples (45.6%), while HPLC detected at least one of the Alternaria mycotoxins within 31 of the PCR positive samples. Detection of Alternaria DNA correlated well with the presence of the analysed Alternaria mycotoxins, indicating that the PCR protocol developed in this work for detection of Alternaria spp. DNA could be used as an indirect marker of the presence of Alternaria mycotoxins in raw and processed tomato products. (c) 2011 Elsevier Ltd. All rights reserved.
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收藏
页码：45 / 52
页数：8
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