Realization of 1 x 106 Theoretical Plates in Liquid Chromatography Using Very Long Pillar Array Columns

被引：92

作者：

De Malsche, Wim ^{[1
]}

De Beeck, Jeff Op ^{[1
]}

De Bruyne, Seim ^{[1
,2
]}

Gardeniers, Han ^{[2
]}

Desmet, Gert ^{[1
]}

机构：

[1] Vrije Univ Brussel, Dept Chem Engn, B-1050 Brussels, Belgium

[2] Mesa Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 03期

关键词：

LOW-DISPERSION TURNS; HIGH-ASPECT-RATIO; PERFORMANCE; SYSTEMS; LC; OPTIMIZATION; SEPARATIONS; FABRICATION; RESOLUTION; DESIGN;

D O I：

10.1021/ac203048n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on the possibility to achieve ultra high efficiencies (order of 1 million theoretical plates) in liquid chromatography in a relatively short time of 20 min (elution time of unretained marker). This was achieved using a micropillar array column with optimized pillar diameter (5 mu m) and interpillar distance (2.5 mu m) to operate close to the Knox and Saleem limit of micropillar array columns in the region of the 1 million theoretical plate mark under the prevailing pressure restriction (350 bar in the present study). The obtained efficiency was slightly affected (some 15 to 20% around the optimal flow rate) by the turns that were inevitably needed to arrange a 3 m long column on a 4 in. silicon wafer.

引用

页码：1214 / 1219

页数：6

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