Enhanced cell uptake of superparamagnetic iron oxide nanoparticles through direct chemisorption of FITC-Tat-PEG600-b-poly(glycerol monoacrylate)

被引：30

作者：

Wang, Chenhong ^{[2
]}

Qiao, Lei ^{[2
]}

Zhang, Quan ^{[2
]}

Yan, Husheng ^{[1
]}

Liu, Keliang ^{[2
]}

机构：

[1] Nankai Univ, Inst Polymer Chem, Minist Educ, Key Lab Funct Polymer Mat, Tianjin 300071, Peoples R China

[2] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2012年 / 430卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Superparamagnetic iron oxide (SPIO) nanoparticle; Multifunctional magnetic nanoparticle system; FITC-Tat penetrating peptide; PENETRATING PEPTIDE; DELIVERY; LIPOSOMES;

D O I：

10.1016/j.ijpharm.2012.04.035

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Magnetic nanoparticles (MNPs) functionalized with specific ligands are emerging as a highly integrated platform for cancer targeting, drug delivery, and magnetic resonance imaging applications. In this study, we describe a multifunctional magnetic nanoparticle system (FITC-Tat MNPs) consisting of a fluorescently labeled cell penetrating peptide (FITC-Tat peptide), a biocompatible block copolymer PEG(600)-b-poly(glycerol monoacrylate) (PEG(600)-b-PGA), and a superparamagnetic iron oxide (SPIO) nanoparticle core. The particles were prepared by direct chemisorption of PEG(600)-b-PGA conjugated with FITC-Tat peptide on the SPIO nanoparticles. FITC-MNPs without Tat were prepared for comparison. Flow cytometry assays revealed significantly higher uptake of FITC-Tat MNPs compared to FITC-MNPs in Caco-2 cells. These results were confirmed using confocal laser scanning microscopy (LSCM), which further demonstrated that the FITC-Tat MNPs accumulated in the cytoplasm and nucleus while the FITC-MNPs were localized in the cell membrane compartments. The FITC-Tat MNPs did not exhibit observable cytotoxicity in MTS assays. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：372 / 380

页数：9

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