Physiological and Proteomic Dissection of the Responses of Two Contrasting Wheat Genotypes to Nitrogen Deficiency

被引:9
|
作者
Karim, Mohammad Rezaul [1 ,2 ]
Wang, Ruonan [1 ,2 ]
Zheng, Lu [1 ]
Dong, Xiaoying [1 ]
Shen, Renfang [1 ]
Lan, Ping [1 ,2 ]
机构
[1] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
美国国家科学基金会;
关键词
wheat; nitrogen starvation; leaf chlorophyll; genes; proteome; ARABIDOPSIS ROOTS REVEALS; AFFINITY NITRATE UPTAKE; ALUMINUM TOLERANCE; GENETIC-VARIATION; USE EFFICIENCY; ACID SOILS; ACIDIFICATION; MECHANISMS; TRAITS; SULFUR;
D O I
10.3390/ijms21062119
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nitrogen deficiency usually occurs along with aluminum toxicity in acidic soil, which is one of the major constraints for wheat production worldwide. In order to compare adaptive processes to N deficiency with different Al-tolerant wheat cultivars, we chose Atlas 66 and Scout 66 to comprehensively analyze the physiological responses to N deficiency, coupled with label-free mass spectrometry-based proteomics analysis. Results showed that both cultivars were comparable in most physiological indexes under N deficient conditions. However, the chlorophyll content in Scout 66 was higher than that of Atlas 66 under N deficiency. Further proteomic analysis identified 5592 and 5496 proteins in the leaves of Atlas 66 and Scout 66, respectively, of which 658 and 734 proteins were shown to significantly change in abundance upon N deficiency, respectively. The majority of the differentially expressed proteins were involved in cellular N compound metabolic process, photosynthesis, etc. Moreover, tetrapyrrole synthesis and sulfate assimilation were particularly enriched in Scout 66. Our findings provide evidence towards a better understanding of genotype-dependent responses under N deficiency which could help us to develop N efficient cultivars to various soil types.
引用
收藏
页数:25
相关论文
共 50 条
  • [1] Comparative proteomic and physiological characterisation of two closely related rice genotypes with contrasting responses to salt stress
    Hosseini, Seyed Abdollah
    Gharechahi, Javad
    Heidari, Manzar
    Koobaz, Parisa
    Abdollahi, Shapour
    Mirzaei, Mehdi
    Nakhoda, Babak
    Salekdeh, Ghasem Hosseini
    FUNCTIONAL PLANT BIOLOGY, 2015, 42 (06) : 527 - 542
  • [2] Proteomic and physiological responses of contrasting two different heat-resistant orchardgrass genotypes to heat stress
    Luo, Dan
    Ding, Qiong
    Ma, Xixi
    Zhu, Jie
    Zou, Kun
    Hong, Wenkai
    Wang, Jin
    Mao, Chunli
    Xie, Zheni
    Wu, Bingchao
    Khan, Imran
    Wang, Xiaoshan
    Feng, Guangyan
    Huang, Linkai
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 245
  • [3] Manganese deficiency in wheat genotypes: Physiological responses and manganese deficiency tolerance index
    Barman, Arijit
    Pandey, Raghu Nath
    Singh, Bhupider
    Das, Bappa
    JOURNAL OF PLANT NUTRITION, 2017, 40 (19) : 2691 - 2708
  • [4] Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance
    Faghani, Elham
    Gharechahi, Javad
    Komatsu, Setsuko
    Mirzaei, Mehdi
    Khavarinejad, Raman Ali
    Najafi, Farzaneh
    Farsad, Laleh Karimi
    Salekdeh, Ghasern Hosseini
    JOURNAL OF PROTEOMICS, 2015, 114 : 1 - 15
  • [5] Comparative physiological and proteomic response to phosphate deficiency between two wheat genotypes differing in phosphorus utilization efficiency
    Zheng, Lu
    Wang, Ruonan
    Zhou, Peijun
    Pan, Yilin
    Shen, Renfang
    Lan, Ping
    JOURNAL OF PROTEOMICS, 2023, 280
  • [6] Molecular Responses to Cadmium Exposure in Two Contrasting Durum Wheat Genotypes
    Sabella, Erika
    Luvisi, Andrea
    Genga, Alessandra
    De Bellis, Luigi
    Aprile, Alessio
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (14)
  • [7] Different responses of two contrasting wheat genotypes to abscisic acid application
    Zhang, X
    Wang, T
    Li, C
    BIOLOGIA PLANTARUM, 2005, 49 (04) : 613 - 616
  • [8] Comparative Physiological and Proteomic Analysis of Two Sugar Beet Genotypes with Contrasting Salt Tolerance
    Wang, Yuguang
    Stevanato, Piergiorgio
    Lv, Chunhua
    Li, Renren
    Geng, Gui
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (21) : 6056 - 6073
  • [9] Physiological Responses of Two Wheat Cultivars to Nitrogen Starvation
    WANG Xiaoli SHAN Yuhua WANG Suhua DU Yan and FENG Ke Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou PRChina Technical Service Center for AgroEnvironmental Safety of Jiangsu Province Yangzhou University Yangzhou PRChina
    Agricultural Sciences in China, 2011, 10 (10) : 1577 - 1585
  • [10] Physiological Responses of Two Wheat Cultivars to Nitrogen Starvation
    Wang Xiao-li
    Shan Yu-hua
    Wang Su-hua
    Du Yan
    Feng Ke
    AGRICULTURAL SCIENCES IN CHINA, 2011, 10 (10): : 1577 - 1585