Assembly and Characterization of Polyelectrolyte Complex Micelles

被引：6
|
作者：
Marras, Alexander E. [1 ]
Vieregg, Jeffrey R. [1 ]
Tirrell, Matthew, V [1 ]
机构：
[1] Univ Chicago, Pritzker Sch Mol Engn, Chicago, IL 60637 USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2020年 / 157期
关键词：
Chemistry; Issue; 157; nucleic acid delivery; polyelectrolyte complex; micelle; nanoparticles; phase separation; oligonucleotides; GENE DELIVERY; PROGRAM; POLYMER; SYSTEMS; DESIGN; BULK;
D O I：
10.3791/60894
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Polyelectrolyte complex micelles (PCMs), core-shell nanoparticles formed by self-assembly of charged polymers in aqueous solution, provide a powerful platform for exploring the physics of polyelectrolyte interactions and also offer a promising solution to the pressing problem of delivering therapeutic oligonucleotides in vivo. Developing predictive structure-property relationships for PCMs has proven difficult, in part due to the presence of strong kinetic traps during nanoparticle self-assembly. This article discusses criteria for choosing polymers for PCM construction and provides protocols based on salt annealing that enable assembly of repeatable, low-polydispersity nanoparticles. We also discuss PCM characterization using light scattering, small-angle X-ray scattering, and electron microscopy.
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页数：12
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