Arachidonic acid activates NADPH oxidase by a direct, calmodulin-regulated mechanism

被引：18

作者：

Hartfield, PJ ^{[1
]}

Robinson, JM ^{[1
]}

机构：

[1] Ohio State Univ, Dept Cell Biol Neurobiol & Anat, Columbus, OH 43210 USA

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 1998年 / 56卷 / 01期

关键词：

arachidonic acid; neutrophils; calmodulin; NADPH; superoxide;

D O I：

10.1016/S0090-6980(98)00036-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In intact neutrophils, arachidonic acid rapidly and transiently activated NADPH oxidase-mediated superoxide generation. Inhibitors of protein kinases (staurosporine and H-7) failed to markedly attenuate arachidonic acid-stimulated superoxide generation. Conversely, the calmodulin antagonist W-7 blocked this arachidonic acid-stimulated response. Similarly, diphenylene iodonium potently inhibited superoxide release. These results suggest that arachidonic acid directly activates the membrane-associated NADPH oxidase and calmodulin and/or calmodulin-binding proteins are required for the assembly of the active oxidase.

引用

页码：1 / 6

页数：6

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