Comprehensive microRNA expression profiling of the hematopoietic hierarchy

被引：125

作者：

Petriv, O. I. ^{[1
,2
]}

Kuchenbauer, F. ^{[4
,5
,6
]}

Delaney, A. D. ^{[7
]}

Lecault, V. ^{[1
]}

White, A. ^{[1
]}

Kent, D. ^{[4
]}

Marmolejo, L. ^{[4
]}

Heuser, M. ^{[4
]}

Berg, T. ^{[4
]}

Copley, M. ^{[4
]}

Ruschmann, J. ^{[4
]}

Sekulovic, S. ^{[4
]}

Benz, C. ^{[4
]}

Kuroda, E. ^{[4
]}

Ho, V. ^{[4
]}

Antignano, F. ^{[4
]}

Halim, T. ^{[4
]}

Giambra, V. ^{[4
]}

Krystal, G. ^{[4
]}

Takei, C. J. F. ^{[4
]}

Weng, A. P. ^{[4
]}

Piret, J. ^{[3
]}

Eaves, C. ^{[4
]}

Marra, M. A. ^{[7
]}

Humphries, R. K. ^{[4
]}

Hansen, C. L. ^{[1
,2
]}

机构：

[1] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V6T 1Z4, Canada

[2] Univ British Columbia, Dept Phys & Astron, Vancouver, BC V6T 1Z4, Canada

[3] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

[4] British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada

[5] Univ Hosp Ulm, Ctr Comprehens Canc, Inst Expt Canc Res, D-89081 Ulm, Germany

[6] Univ Hosp Ulm, Dept Internal Med 3, D-89081 Ulm, Germany

[7] British Columbia Canc Agcy, Genome Sci Ctr, Vancouver, BC V5Z 4E6, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 35期

关键词：

RT-qPCR; stem cell; hematopoiesis; microfluidic; single cell; STEM-CELLS; PROGENITOR; DIFFERENTIATION; TRANSCRIPTOME;

D O I：

10.1073/pnas.1009320107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The hematopoietic system produces a large number of highly specialized cell types that are derived through a hierarchical differentiation process from a common stem cell population. miRNAs are critical players in orchestrating this differentiation. Here, we report the development and application of a high-throughput microfluidic real-time quantitative PCR (RT-qPCR) approach for generating global miRNA profiles for 27 phenotypically distinct cell populations isolated from normal adult mouse hematopoietic tissues. A total of 80,000 RT-qPCR assays were used to map the landscape of miRNA expression across the hematopoietic hierarchy, including rare progenitor and stem cell populations. We show that miRNA profiles allow for the direct inference of cell lineage relations and functional similarity. Our analysis reveals a close relatedness of the miRNA expression patterns in multipotent progenitors and stem cells, followed by a major reprogramming upon restriction of differentiation potential to a single lineage. The analysis of miRNA expression in single hematopoietic cells further demonstrates that miRNA expression is very tightly regulated within highly purified populations, underscoring the potential of single-cell miRNA profiling for assessing compartment heterogeneity.

引用

页码：15443 / 15448

页数：6

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