Effects of grape seed extract on oxidative stress and antioxidant defense markers in streptozotocin-induced diabetic rats

被引：10
作者：
Belviranli, Muaz [1 ]
Gokbel, Hakki [1 ]
Okudan, Nilsel [1 ]
Buyukbas, Sadik [2 ]
机构：
[1] Selcuk Univ, Dept Physiol, Fac Med, Konya, Turkey
[2] Necmettin Erbakan Univ, Dept Biochem, Meram Fac Med, Konya, Turkey
来源：
TURKISH JOURNAL OF MEDICAL SCIENCES | 2015年 / 45卷 / 03期
关键词：
Experimental diabetes; grape seed extract; oxidative stress; antioxidant enzyme activity; adenosine deaminase; nitric oxide; ADENOSINE-DEAMINASE ACTIVITY; CENTRAL-NERVOUS-SYSTEM; ACID PHENETHYL ESTER; PROANTHOCYANIDIN EXTRACT; LIPID-PEROXIDATION; PERIPHERAL NEUROPATHY; NITRIC-OXIDE; PROTECTION; SERUM; EXPRESSION;
D O I：
10.3906/sag-1404-5
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background/aim: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg(-1) day(-1)) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed. Results: Both XO and ADA activities increased and NO levels decreased in diabetic rats (P < 0.05). GSE supplementation normalized all of these changes. Antioxidant enzyme activities decreased in diabetic rats compared to the controls (P < 0.05). GSE supplementation increased antioxidant enzyme activities in both diabetic and healthy rats (P < 0.05). Conclusion: These findings suggest that 6 weeks of oral GSE supplementation may prevent oxidative stress and improve antioxidant status in diabetic rats.
引用
收藏
页码：489 / 495
页数：7
相关论文
共 54 条
[1]   Evaluation of some biochemical changes in diabetic patients [J].
Abou-Seif, MA ;
Youssef, AA .
CLINICA CHIMICA ACTA, 2004, 346 (02) :161-170
[2]   Antidiabetic activity of red wine polyphenolic extract, ethanol, or both in streptozotocin-treated rats [J].
Al-Awwadi, N ;
Azay, J ;
Poucheret, P ;
Cassanas, G ;
Krosniak, M ;
Auger, C ;
Gasc, F ;
Rouanet, JM ;
Cros, G ;
Teissèdre, PL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) :1008-1016
[3]   Amelioration of streptozotocin-induced diabetes mellitus, oxidative stress and dyslipidemia in rats by tomato extract lycopene [J].
Ali, Mamdouh M. ;
Agha, Fatma G. .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2009, 69 (03) :371-379
[4]   Central nervous system protection by resveratrol in streptozotocin-induced diabetic rats [J].
Ates, Ozkan ;
Cayli, Suleyman R. ;
Yucel, Neslihan ;
Altinoz, Eyup ;
Kocak, Ayhan ;
Durak, M. Akif ;
Turkoz, Yusuf ;
Yologlu, Saim .
JOURNAL OF CLINICAL NEUROSCIENCE, 2007, 14 (03) :256-260
[5]   Cellular protection with proanthocyanidins derived from grape seeds [J].
Bagchi, D ;
Bagchi, M ;
Stohs, SJ ;
Ray, SD ;
Sen, CK ;
Preuss, HG .
ALCOHOL AND WINE IN HEALTH AND DISEASE, 2002, 957 :260-270
[6]  
Bagchi D, 1997, RES COMMUN MOL PATH, V95, P179
[7]   Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats [J].
Balu, M ;
Sangeetha, P ;
Murali, G ;
Panneerselvam, C .
BRAIN RESEARCH BULLETIN, 2006, 68 (06) :469-473
[8]   Role of oxidative stress in diabetic complications - A new perspective on an old paradigm [J].
Baynes, JW ;
Thorpe, SR .
DIABETES, 1999, 48 (01) :1-9
[9]  
Çay M, 2001, RES EXP MED, V200, P205
[10]   Caffeic acid phenethyl ester (CAPE) protects brain against oxidative stress and inflammation induced by diabetes in rats [J].
Celik, Sefa ;
Erdogan, Suat .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2008, 312 (1-2) :39-46
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