Extensive characterization of Campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization

被引：18
作者：
Thibodeau, Alexandre [1 ,2 ,3 ]
Fravalo, Philippe [1 ,2 ,3 ]
Taboada, Eduardo N. [5 ]
Laurent-Lewandowski, Sylvette [1 ,2 ]
Guevremont, Evelyne [3 ,4 ]
Quessy, Sylvain [1 ,2 ,3 ]
Letellier, Ann [1 ,2 ,3 ]
机构：
[1] Univ Montreal, Fac Vet Med, Dept Pathol & Microbiol, NSERC Ind Res Chair Meat Safety CRSV, St Hyacinthe, PQ, Canada
[2] Univ Montreal, Fac Vet Med, Dept Pathol & Microbiol, Swine & Avian Infect Dis Res Ctr CRIPA, St Hyacinthe, PQ, Canada
[3] Univ Montreal, Fac Vet Med, Dept Pathol & Microbiol, Grp Rech & Enseignement Salubrite Alimentaire GRE, St Hyacinthe, PQ, Canada
[4] Agr & Agri Food Canada, Food Res & Dev Ctr, St Hyacinthe, PQ, Canada
[5] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Lethbridge, AB, Canada
来源：
BMC MICROBIOLOGY | 2015年 / 15卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
Campylobacter jejuni; Chicken; Colonization; CGF typing; Autoagglutination; Chemotaxis; Adhesion and invasion; Competition; STRAINS; IDENTIFICATION; RESISTANCE;
D O I：
10.1186/s12866-015-0433-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background: Campylobacter jejuni is responsible for human foodborne enteritis. This bacterium is a remarkable colonizer of the chicken gut, with some strains outcompeting others for colonization. To better understand this phenomenon, the objective of this study was to extensively characterize the phenotypic performance of Campylobacter jejuni chicken strains and associate their gut colonizing ability with specific genes. Results: Campylobacter jejuni isolates (n = 45) previously analyzed for the presence of chicken colonization associated genes were further characterized for phenotypic properties influencing colonization: autoagglutination and chemotaxis as well as adhesion to and invasion of primary chicken caecal cells. This allowed strains to be ranked according to their in vitro performance. After their in vitro capacity to outcompete was demonstrated in vivo, strains were then typed by comparative genomic fingerprinting (CGF). In vitro phenotypical properties displayed a linear variability among the tested strains. Strains possessing higher scores for phenotypical properties were able to outcompete others during chicken colonization trials. When the gene content of strains was compared, some were associated with different phenotypical scores and thus with different outcompeting capacities. Use of CGF profiles showed an extensive genetic variability among the studied strains and suggested that the outcompeting capacity is not predictable by CGF profile. Conclusion: This study revealed a wide array of phenotypes present in Campylobacter jejuni strains, even though they were all recovered from chicken caecum. Each strain was classified according to its in vitro competitive potential and its capacity to compete for chicken gut colonization was associated with specific genes. This study also exposed the disparity existing between genetic typing and phenotypical behavior of Campylobacter jejuni strains.
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页数：14
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