Menkes protein localization in rat parotid acinar cells

被引：6
作者：
D'Amico, F
Skarmoutsou, E
Sanfilippo, S
Camakaris, J
机构：
[1] Univ Catania, CMEM Med Electron Microscopy Unit, I-95124 Catania, Italy
[2] Univ Melbourne, Dept Genet, Parkville, Vic 3010, Australia
来源：
ACTA HISTOCHEMICA | 2005年 / 107卷 / 05期
关键词：
menkes protein; parotid gland; acinar cells; immunocyto-; chemistry; ultrastructure;
D O I：
10.1016/j.acthis.2005.07.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The aim was to study the subcellular localization of the (MNK; ATP7A) in the rat parotid acinar cell. MNK protein is a copper transporting P-type ATPase whose absence or dysfunction causes a fatal neurodegenerative disorder, MNK disease. Rat parotid glands were fixed and tow-temperature embedded in Lowicryl K4M resin, and ultrathin sections were prepared for immunocytochemical. analysis. Immunolocatization of MNK was demonstrated mainly over the trans Golgi network (TGN) area. Immature and mature secretory granules were also labelled, indicating that MNK protein could be involved here in copper secretion from acinar cells into saliva, consistent with a proposed cariostatic role for copper. (c) 2005 Elsevier GmbH. All rights reserved.
引用
收藏
页码：373 / 378
页数：6
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