Differential behaviour of the dinitrosalicylic acid (DNS) reagent towards mono- and di-saccharide sugars

被引：210

作者：

Saqib, Abdul Aala Najmus ^{[1
]}

Whitney, Philip John ^{[1
]}

机构：

[1] Univ Surrey, Fac Hlth & Med Sci, Guildford GU2 7XH, Surrey, England

来源：

BIOMASS & BIOENERGY | 2011年 / 35卷 / 11期

关键词：

DNS; Reducing sugars; Lignocellulose; Mono-saccharides; Di-saccharides; FILTER-PAPER ASSAY; CELLULASE ACTIVITY; HYDROLYSIS; ENZYMES;

D O I：

10.1016/j.biombioe.2011.09.013

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

3,5-Dinitrosalicylic acid (DNS) reagent is widely used in the estimation of reducing sugars. The reagent shows a differential behaviour towards mono- and di-saccharides. This phenomenon has been misinterpreted in the literature. Contrary to the facts, it has been reported that the DNS test is less sensitive for the estimation of cellobiose than it is for the estimation of glucose. This communication clarifies the concept. In addition, the study also compares the reaction of different mono- and di-saccharides and discusses the difference in their reactivity. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：4748 / 4750

页数：3

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