Detection of protein-protein interactions in vivo based on protein splicing

被引：39

作者：

Ozawa, T ^{[1
]}

Umezawa, Y

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Corp, Tokyo, Japan

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 05期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1016/S1367-5931(00)00244-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mammalian cells, protein-protein interactions constitute essential regulatory steps that modulate the activity of signaling pathways. In recent years, several approaches towards understanding the interactions have been developed. We describe herein a new method for detecting protein-protein interactions in vivo based on protein splicing and highlight some potential applications of this technique.

引用

页码：578 / 583

页数：6

共 36 条

[1] Improved efficiency Sos recruitment system: expression of the mammalian GAP reduces isolation of Ras GTPase false positives
Aronheim, A
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (16) : 3373 - 3374
[2] Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions
Aronheim, A
Zandi, E
Hennemann, H
Elledge, SJ
Karin, M
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) : 3094 - 3102
[3] Protein recruitment systems for the analysis of protein-protein interactions
Aronheim, A
[J]. BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) : 1009 - 1013
[4] Epidermal growth factor receptor dimerization monitored in live cells
Blakely, BT
Rossi, FMV
Tillotson, B
Palmer, M
Estelles, A
Blau, HM
[J]. NATURE BIOTECHNOLOGY, 2000, 18 (02) : 218 - 222
[5] The Ras recruitment system, a novel approach to the study of protein-protein interactions
Broder, YC
Katz, S
Aronheim, A
[J]. CURRENT BIOLOGY, 1998, 8 (20) : 1121 - 1124
[6] Biosynthesis of a head-to-tail cyclized protein with improved biological activity
Camarero, JA
Muir, TW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (23) : 5597 - 5598
[7] THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST
CHIEN, CT
BARTEL, PL
STERNGLANZ, R
FIELDS, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) : 9578 - 9582
[8] Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
Chong, SR
Mersha, FB
Comb, DG
Scott, ME
Landry, D
Vence, LM
Perler, FB
Benner, J
Kucera, RB
Hirvonen, CA
Pelletier, JJ
Paulus, H
Xu, MQ
[J]. GENE, 1997, 192 (02) : 271 - 281
[9] Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes
Conti, E
Franks, NP
Brick, P
[J]. STRUCTURE, 1996, 4 (03) : 287 - 298
[10] Insertion of a synthetic peptide into a recombinant protein framework: A protein biosensor
Cotton, GJ
Ayers, B
Xu, R
Muir, TW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (05) : 1100 - 1101

← 1 2 3 4 →