Prevalence and molecular characterization of bovine Cryptosporidium isolates in India

被引:37
作者
Paul, S. [1 ]
Chandra, D. [1 ]
Ray, D. D. [1 ]
Tewari, A. K. [1 ]
Rao, J. R. [1 ]
Banerjee, P. S. [1 ]
Baidya, S. [1 ]
Raina, O. K. [1 ]
机构
[1] Indian Vet Res Inst, Div Parasitol, Parasitol Lab, Izatnagar 243122, Uttar Pradesh, India
关键词
Cryptosporidium; 18S SSU rRNA; nested PCR; PCR-RFLP;
D O I
10.1016/j.vetpar.2008.01.044
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A survey based on PCR assay of 18S SSU rRNA gene revealed a 30.2% infection with Cryptosporidium spp., out of 457 faecal samples collected from neonatal bovine calves across three different regions of India. The PCR-RFLP pattern of the gene in all the positive cases established the species as Cryptosporidium parvum. Highest prevalence was recorded in the monsoon months (37.3%) and in the calves showing acute diarrhoea (32.3%). The calves below 15 days of age were mostly affected (45.1%). The infection was more prevalent in the northern parts (35.4%) of the country than in the eastern or southern parts. Results indicated that C. parvum was the only species of Cryptosporidium prevalent in bovine calves in three different geographical regions of India. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:143 / 146
页数:4
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