Selective detection of uric acid in the presence of ascorbic acid at physiological pH by using a β-cyclodextrin modified copolymer of sulfanilic acid and N-acetylaniline

被引：35
作者：
Wu, Shouguo [1 ]
Wang, Taoling [1 ]
Gao, Zongyong [2 ]
Xu, Haihong [3 ]
Zhou, Baineng [1 ]
Wang, Chuanqin [1 ]
机构：
[1] Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
[2] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China
[3] Hefei Third Peoples Hosp, Clin Lab, Hefei 230022, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2008年 / 23卷 / 12期
基金：
中国国家自然科学基金;
关键词：
uric acid; ascorbic acid; beta-cyclodextrin; chemically modified electrode; electrocatalysis;
D O I：
10.1016/j.bios.2008.02.012
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A beta-cyclodextrin (CD) modified copolymer membrane of sulfanilic acid (p-ASA) and N-acetylaniline (SPNAANI) on glassy carbon electrode (GCE) was prepared and used to determine uric acid (UA) in the presence of a large excess of ascorbic acid (AA) by differential pulse voltammetry (DPV). The properties of the copolymer were characterized by X-ray photoelectron spectra (XPS) and Raman spectroscopy. The oxidation peaks of AA and UA were well separated at the composite membrane modified electrode in phosphate buffer solution (PBS, pH 7.4). A linear relationship between the peak current and the concentration of UA was obtained in the range from 1.0 x 10(-5) to 3.5 x 10(-4) mol L-1, and the detection limit was 2.7 x 10(-6) mol L-1 at a signal-to-noise ratio of 3. Two hundred and fifty-fold excess of AA did not interfere with the determination of UA. The application of the prepared electrode was demonstrated by measuring UA in human serum samples without any pretreatment, and the results were comparatively in agreement with the spectrometric clinical assay method. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：1776 / 1780
页数：5
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