Transcription of Unnatural Fluorescent Nucleotides and their Application with Graphene Oxide for the Simple and Direct Detection of miRNA

被引：9

作者：

Le, Binh Huy ^{[1
]}

Seo, Young Jun ^{[1
,2
]}

机构：

[1] Chonbuk Natl Univ, Dept Bioact Mat Sci, Jeonju 561756, South Korea

[2] Chonbuk Natl Univ, Dept Chem, Jeonju 561756, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2018年 / 39卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

Probe; Fluorescent nucleotides; Transcription; miRNA; Graphene oxide; ROLLING CIRCLE AMPLIFICATION; NUCLEIC-ACID HYBRIDIZATION; MOLECULAR BEACONS; DNA; MICRORNAS;

D O I：

10.1002/bkcs.11549

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study we synthesized two differently sized fluorescent RNA nucleotides, rUthioTP and rUpyrTP, and examined their transcription ability using T7 RNA polymerase. The smaller rUthioTP could be incorporated and extended to produce a corresponding RNA sequence, but rUpyrTP could not. We then used this rUthioTP-containing fluorogenic transcription system, in conjunction with graphene oxide(GO), for the detection of miRNA 146a with high sensitivity and selectivity. This combination of a transcribed RNA product and GO is a simple in situ probing system for the detection of miRNA 146a-one that is less time consuming and more cost-effective.

引用

页码：1054 / 1057

页数：4

共 31 条

[1] microRNAs: Tiny regulators with great potential [J].

Ambros, V .

CELL, 2001, 107 (07) :823-826

[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3] DETECTION OF NUCLEIC-ACID HYBRIDIZATION BY NONRADIATIVE FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].

CARDULLO, RA ;

AGRAWAL, S ;

FLORES, C ;

ZAMECNIK, PC ;

WOLF, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8790-8794

[4] Pyrene excimer signaling molecular beacons for probing nucleic acids [J].

Conlon, Patrick ;

Yang, Chaoyong James ;

Wu, Yanrong ;

Chen, Yan ;

Martinez, Karen ;

Kim, Youngmi ;

Stevens, Nathan ;

Marti, Angel A. ;

Jockusch, Steffen ;

Turro, Nicholas J. ;

Tan, Weihong .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (01) :336-342

[5] Escherichia coli lipopolysaccharides and Staphylococcus aureus enterotoxin B differentially modulate inflammatory microRNAs in bovine monocytes [J].

Dilda, Francesca ;

Gioia, Gloria ;

Pisani, Laura ;

Restelli, Laura ;

Lecchi, Cristina ;

Albonico, Francesca ;

Bronzo, Valerio ;

Mortarino, Michele ;

Ceciliani, Fabrizio .

VETERINARY JOURNAL, 2012, 192 (03) :514-516

[6]

Gait M.J., 1984, OLIGONUCLEOTIDE SYNT, P1

[7] DETECTION OF SPECIFIC POLYMERASE CHAIN-REACTION PRODUCT BY UTILIZING THE 5'-]3' EXONUCLEASE ACTIVITY OF THERMUS-AQUATICUS DNA-POLYMERASE [J].

HOLLAND, PM ;

ABRAMSON, RD ;

WATSON, R ;

GELFAND, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7276-7280

[8] Fluorometric Detection of MicroRNA Using Isothermal Gene Amplification and Graphene Oxide [J].

Hong, Chaesun ;

Baek, Ahruem ;

Hah, Sang Soo ;

Jung, Woong ;

Kim, Dong-Eun .

ANALYTICAL CHEMISTRY, 2016, 88 (06) :2999-3003

[9] A highly discriminating quencher-free molecular beacon for probing DNA [J].

Hwang, GT ;

Seo, YJ ;

Kim, BH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (21) :6528-6529

[10] Single excitation three color folded DNA probe for SNP typing [J].

Joo, Han Na ;

Seo, Young Jun .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2015, 25 (22) :5286-5290

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