Comparative Lipidomic Profiling of Xylose-Metabolizing S. cerevisiae and Its Parental Strain in Different Media Reveals Correlations Between Membrane Lipids and Fermentation Capacity

被引：23

作者：

Xia, Jinmei ^{[1
,2
]}

Jones, A. Daniel ^{[3
,4
,5
]}

Lau, Ming W. ^{[6
]}

Yuan, Ying-Jin ^{[1
,2
]}

Dale, Bruce E. ^{[3
,6
]}

Balan, Venkatesh ^{[3
,6
]}

机构：

[1] Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Dept Pharmaceut Engn, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

[3] Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA

[4] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[5] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

[6] Michigan State Univ, Dept Chem Engn & Mat Sci, BCRL, Lansing, MI 48910 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2011年 / 108卷 / 01期

基金：

中国国家自然科学基金;

关键词：

yeast; lipidome analysis; LC-MS/MS; AFEX; xylose; cellulosic ethanol; TREATED CORN STOVER; SACCHAROMYCES-CEREVISIAE; YEAST; ETHANOL; PHOSPHATIDYLSERINE; ADAPTATION; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLINOSITOL; BIOSYNTHESIS; FLUIDITY;

D O I：

10.1002/bit.22910

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phospholipids (PLs) serve as the foundation for structure and function in most cell membranes. In order to reveal the correlations between PLs composition and fermentation performance of cells, a comparative lipidomics study was carried out using a recombinant xylose fermenting yeast strain Saccharomyces cerevisiae 424A(LNH-ST) and its parental strain 4124. Profiling of yeast lipids was performed using ultra performance liquid chromatography (UPLC)-MS/MS, leading to identification of 123 PL species. PL compositions were determined for both strains grown in rich medium(yeast extract peptone), limited medium (yeast nitrogen base), and ammonia fiber expansion pretreated corn stover hydrolysate. Principal component analysis of lipidomic data revealed that the PL profile for both strains varied significantly depending upon cultivating media composition. Further analysis of different classes of PLs revealed that the phosphatidylinositol/phosphatidylserine (PI/PS) ratio was closely related to cell growth rates. Both strains possessed higher phosphatidylcholine (PC) levels at an expense of phosphatidylethanolamine (PE) levels when entering stationary phase and the PC/PE ratios showed consistency with glucose utilization rates. Interestingly, PI synthesis lagged behind when available nutrients were limited, and PI levels were closely correlated with xylose metabolism. Biotechnol. Bioeng. 2011;108: 12-21. (C) 2010 Wiley Periodicals, Inc.

引用

页码：12 / 21

页数：10