Measurements of the effect of membrane asymmetry on the mechanical properties of lipid bilayers

被引：95

作者：

Elani, Yuval ^{[1
]}

Purushothaman, Sowmya ^{[1
]}

Booth, Paula J. ^{[2
]}

Seddon, John M. ^{[1
]}

Brooks, Nicholas J. ^{[1
]}

Law, Robert V. ^{[1
]}

Ces, Oscar ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Kings Coll London, Dept Chem, London SE1 1DB, England

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 32期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

CTP-PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE; GIANT UNILAMELLAR VESICLES; BENDING RIGIDITY; SPONTANEOUS CURVATURE; ARTIFICIAL CELL; IN-VITRO; PROTEIN; CHANNEL; MODULATION; FLIPPASES;

D O I：

10.1039/c5cc00712g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We detail an approach for constructing asymmetric membranes and characterising their mechanical properties, leading to the first measurement of the effect of asymmetry on lipid bilayer mechanics. Our results demonstrate that asymmetry induces a significant increase in rigidity compared to symmetric membranes. Given that all biological membranes are asymmetric our findings have profound implications for the role of this phenomenon in biology.

引用

收藏

页码：6976 / 6979

页数：4

相关论文

共 52 条

[1] Modulation of CTP:phosphocholine cytidylyltransferase by membrane torque tension [J].

Attard, GS ;

Smith, WS ;

Templer, RH ;

Hunt, AN ;

Jackowski, S .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1998, 26 (03) :S230-S230

[2] Modulation of CTP:phosphocholine cytidylyltransferase by membrane curvature elastic stress [J].

Attard, GS ;

Templer, RH ;

Smith, WS ;

Hunt, AN ;

Jackowski, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) :9032-9036

[3] ArfGAP1 responds to membrane curvature through the folding of a lipid packing sensor motif [J].

Bigay, J ;

Casella, JF ;

Drin, G ;

Mesmin, B ;

Antonny, B .

EMBO JOURNAL, 2005, 24 (13) :2244-2253

[4] Chemical control of phospholipid distribution across bilayer membranes [J].

Boon, JM ;

Smith, BD .

MEDICINAL RESEARCH REVIEWS, 2002, 22 (03) :251-281

[5] Membrane protein folding makes the transition [J].

Booth, Paula J. ;

Clarke, Jane .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) :3947-3948

[6] Same in the membrane: designing systems to modulate membrane proteins [J].

Booth, PJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2005, 15 (04) :435-440

[7] Evidence that bilayer bending rigidity affects membrane protein folding [J].

Booth, PJ ;

Riley, ML ;

Flitsch, SL ;

Templer, RH ;

Farooq, A ;

Curran, AR ;

Chadborn, N ;

Wright, P .

BIOCHEMISTRY, 1997, 36 (01) :197-203

[8] In vitro studies of membrane protein folding [J].

Booth, PJ ;

Templer, RH ;

Meijberg, W ;

Allen, SJ ;

Curran, AR ;

Lorch, M .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2001, 36 (06) :501-603

[9] Unravelling the folding of bacteriorhodopsin [J].

Booth, PJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2000, 1460 (01) :4-14

[10] Giant Vesicles "Colonies": A Model for Primitive Cell Communities [J].

Carrara, Paolo ;

Stano, Pasquale ;

Luisi, Pier Luigi .

CHEMBIOCHEM, 2012, 13 (10) :1497-1502

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