Thioether bond formation by SPASM domain radical SAM enzymes: Cα H-atom abstraction in subtilosin A biosynthesis

被引：50

作者：

Benjdia, Alhosna ^{[1
]}

Guillot, Alain ^{[1
]}

Lefranc, Benjamin ^{[2
,3
]}

Vaudry, Hubert ^{[2
,3
]}

Leprince, Jerome ^{[2
,3
]}

Berteau, Olivier ^{[1
]}

机构：

[1] Univ Paris Saclay, ChemSyBio, INRA, Micalis Inst,AgroParisTech, F-78350 Jouy En Josas, France

[2] INSERM U982, F-76821 Mont St Aignan, France

[3] Univ Rouen, Inst Res & Innovat Biomed, Reg Platform Cell Imaging, PRIMACEN, F-76821 Mont St Aignan, France

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 37期

基金：

欧洲研究理事会;

关键词：

SULFATASE-MATURATING ENZYMES; POSTTRANSLATIONAL MODIFICATION; BACILLUS-SUBTILIS; PROKARYOTES; SUBSTRATE; REVEALS; SULFUR; LYASE; COLI;

D O I：

10.1039/c6cc01317a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AlbA is a radical SAM enzyme catalyzing the formation of three unusual thioether bonds in the antibiotic subtilosin A. We demonstrate here that AlbA catalyzes direct C-alpha H-atom abstraction and likely contains three essential [4Fe-4S] centers. This leads us to propose novel mechanistic perspectives for thioether bond catalysis by radical SAM enzymes.

引用

页码：6249 / 6252

页数：4

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