Time-resolved detection of luminescence for electrophoretic methods

被引:4
|
作者
Dodgson, Bryan J. [1 ,2 ]
Krylov, Sergey N. [1 ,2 ]
机构
[1] York Univ, Dept Chem, Toronto, ON M3J 2R7, Canada
[2] York Univ, Ctr Res Biomol Interact, Toronto, ON M3J 2R7, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Capillary-gel electrophoresis; Capillary-zone electrophoresis; Electrophoresis; Excitation wavelength; Fluorescence; Instrumentation design; Limit of detection; Microchip electrophoresis; Slab-gel electrophoresis; Time-resolved detection; FLUORESCENCE LIFETIME DETECTION; LASER-INDUCED FLUORESCENCE; CAPILLARY-ZONE-ELECTROPHORESIS; DNA RESTRICTION FRAGMENTS; NEAR-IR FLUORESCENCE; EQUILIBRIUM MIXTURES; MULTIPLEXED FLUORESCENCE; GEL-ELECTROPHORESIS; SMART APTAMERS; PHOSPHORESCENCE;
D O I
10.1016/j.trac.2011.10.007
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Time-resolved luminescence has been employed as a method of detection for electrophoretic studies for nearly two decades. We discuss experiments conducted on capillary-zone electrophoresis, capillary-gel electrophoresis, slab-gel electrophoresis and microchip electrophoresis. We place emphasis on the variety of sample molecules, and applications in order to show the versatility of this technique. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:23 / 34
页数:12
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