Infectivity studies of influenza virus hemagglutinin receptor binding site mutants in mice

被引：21

作者：

Meisner, Jeffrey

Szretter, Kristy J. ^{[2
]}

Bradley, Konrad C.

Langley, William A.

Li, Zhu-Nan

Lee, Byeong-Jae

Thoennes, Sudha

Martin, Javier ^{[3
]}

Skehel, John J. ^{[4
]}

Russell, Rupert J. ^{[5
]}

Katz, Jacqueline M. ^{[2
]}

Steinhauer, David A. ^{[1
]}

机构：

[1] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA

[2] CDC, Influenza Branch MS G16, Atlanta, GA 30333 USA

[3] Natl Inst Biol Stand & Controls, Blanche Lane, Div Virol, Potters Bar EN6 3QG, Herts, England

[4] Natl Inst Med Res, London NW7 1AA, England

[5] Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, Scotland

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 10期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JVI.01958-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.

引用

页码：5079 / 5083

页数：5

共 40 条

[21] The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties [J].