Infectivity studies of influenza virus hemagglutinin receptor binding site mutants in mice

被引：21
作者：
Meisner, Jeffrey
Szretter, Kristy J. [2 ]
Bradley, Konrad C.
Langley, William A.
Li, Zhu-Nan
Lee, Byeong-Jae
Thoennes, Sudha
Martin, Javier [3 ]
Skehel, John J. [4 ]
Russell, Rupert J. [5 ]
Katz, Jacqueline M. [2 ]
Steinhauer, David A. [1 ]
机构：
[1] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA
[2] CDC, Influenza Branch MS G16, Atlanta, GA 30333 USA
[3] Natl Inst Biol Stand & Controls, Blanche Lane, Div Virol, Potters Bar EN6 3QG, Herts, England
[4] Natl Inst Med Res, London NW7 1AA, England
[5] Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, Scotland
来源：
JOURNAL OF VIROLOGY | 2008年 / 82卷 / 10期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1128/JVI.01958-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The replicative properties of influenza virus hemagglutinin (RA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.
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收藏
页码：5079 / 5083
页数：5
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