Increased expression of MUC5AC and MUC5B promoting bacterial biofilm formation in chronic rhinosinusitis patients

被引：20
作者：
Mao, Yong-Jun [1 ]
Chen, Hai-Hong [1 ]
Wang, Bo [2 ]
Liu, Xiang [1 ]
Xiong, Gao-Yun [3 ]
机构：
[1] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Head Neck Otolaryngol, Hangzhou 310003, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Pathol, Hangzhou 310003, Zhejiang, Peoples R China
[3] TongDe Hosp Zhejiang Prov, Dept Head Neck Otolaryngol, Hangzhou 310012, Zhejiang, Peoples R China
来源：
AURIS NASUS LARYNX | 2015年 / 42卷 / 04期
关键词：
Chronic rhinosinusitis; MUC1; MUC2; MUC5AC; MUC5B; Bacterial biofilm; MUCIN GENES; MESSENGER-RNA; UP-REGULATION; BINDING; CELLS; STREPTOCOCCI; ADHERENCE; PATTERNS; MUCOSA;
D O I：
10.1016/j.anl.2014.12.004
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Objective: Binding of bacteria to mucin and then colonizing is the first step of bacterial biofilm (BBF) formation in chronic rhinosinusitis (CRS) patients. Yet, information is sparse on how mucins effects on BBF formation. In this study, we aimed to investigate the relationship between mucin expression and the formation of BBF in CRS patients. Methods: Sinus mucosa were harvested from 24 patients undergoing endoscopic surgery for CRS. The positive of BBF formation were detected by scanning electronic microscopy (SEM) and the expression of MUC1, MUC2, MUC5AC, and MUC5B were determined by immunohistochemistry (IHC). The difference expression of mucins were analyzed between the BBF positive and negative cohorts in CRS patients. Results: MUC5AC and MUC5B are two major mucins in CRS mucosa with the former mainly restricted to the goblet cells of epithelium and the latter mainly restricted to the submucosal glands. Expression of MUC5AC and MUC5B in the sinus mucosa of BBF(+) CRS group was significantly higher than those in BBF(-) CRS group (p < 0.05). For MUC1 and MUC2, no significant difference was found between the two groups (p > 0.05). Conclusion: Our study indicated that increased expression of MUC5AC and MUC5B may play an important role in the pathogenesis of BBF formation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：294 / 298
页数：5
相关论文
共 27 条
[1]   Major secretory mucin expression in chronic sinusitis [J].
Ali, MS ;
Hutton, DA ;
Wilson, JA ;
Pearson, JP .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2005, 133 (03) :423-428
[2]   Bacterial biofilms in chronic rhinosinusitis and their relationship with inflammation severity [J].
Chen, Hai-Hong ;
Liu, Xiang ;
Ni, Chao ;
Lu, Yin-Ping ;
Xiong, Gao-Yun ;
Lu, Yu-Yu ;
Wang, Shen-Qing .
AURIS NASUS LARYNX, 2012, 39 (02) :169-174
[3]   The expression of MUC5AC and MUC5B mucin genes in the mucosa of chronic rhinosinusitis and nasal polyposis [J].
Ding, Guo Qiang ;
Zheng, Chun Quan .
AMERICAN JOURNAL OF RHINOLOGY, 2007, 21 (03) :359-366
[4]   Neutrophil elastase induces MUC5AC messenger RNA expression by an oxidant-dependent mechanism [J].
Fischer, B ;
Voynow, J .
CHEST, 2000, 117 (05) :317S-320S
[5]   Role of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis [J].
Foreman, Andrew ;
Boase, Sam ;
Psaltis, Alkis ;
Wormald, Peter-John .
CURRENT ALLERGY AND ASTHMA REPORTS, 2012, 12 (02) :127-135
[6]   Do Biofilms Contribute to the Initiation and Recalcitrance of Chronic Rhinosinusitis? [J].
Foreman, Andrew ;
Jervis-Bardy, Joshua ;
Wormald, Peter-John .
LARYNGOSCOPE, 2011, 121 (05) :1085-1091
[7]   Biofilms with fungi in chronic rhinosinusitis [J].
Healy, David Y. ;
Leid, Jeff G. ;
Sanderson, Alicia R. ;
Hunsaker, Darrell H. .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2008, 138 (05) :641-647
[8]  
Huang Xuekun, 2007, Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi, V21, P486
[9]   Expression of MUC5AC mRNA in the goblet cells of human nasal mucosa [J].
Kim, CH ;
Song, KS ;
Kim, SS ;
Kim, HU ;
Seong, JK ;
Yoon, JH .
LARYNGOSCOPE, 2000, 110 (12) :2110-2113
[10]   Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis [J].
Kim, DH ;
Chu, HS ;
Lee, JY ;
Hwang, SJ ;
Lee, SH ;
Lee, HM .
ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY, 2004, 130 (06) :747-752
123