Effect of different medium and sucrose concentrations on germination of somatic embryos in grape

被引:4
|
作者
Elidemir, A. Y. [1 ]
Uzun, H. J. [1 ]
Bayir, A. [1 ]
机构
[1] Akdeniz Univ, Fac Agr, Dept Hort, TR-07070 Antalya, Turkey
关键词
Vitis vinifera; in vitro culture; culture medium; embryo stages; germination;
D O I
10.17660/ActaHortic.2007.754.15
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The study was aimed to obtain effect of different medium and sucrose concentrations on germination of somatic embryos of grape. Direct somatic embryogenesis from cotyledone of grape hybrid was obtained in E20A medium supplemented with 0.01 mg/L IAA. Somatic embryos were cultured in different stages (globular, heart and torpedo). They were germinated in different medium (E20A, N&N and C medium) and different sucrose concentrations (10, 30, 50 and 70 g/L). The germination rate ranged from 23% to 95% in all medium.
引用
收藏
页码:117 / 121
页数:5
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