Analysis of interferon gamma protein expression in zebrafish (Danio rerio)

被引：17

作者：

Yoon, Sohye ^{[1
]}

Alnabulsi, Ayham ^{[2
]}

Wang, Ting Yu ^{[1
,3
]}

Lee, Po Tsang ^{[1
,5
]}

Chen, Tzong-Yueh ^{[3
]}

Bird, Steve ^{[1
,4
]}

Zou, Jun ^{[1
]}

Secombes, Christopher J. ^{[1
]}

机构：

[1] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB9 1FX, Scotland

[2] Vertebrate Antibodies Ltd, Aberdeen, Scotland

[3] Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Mol Genet Lab, Tainan 701, Taiwan

[4] Univ Waikato, Mol Genet, Dept Biol Sci, Hamilton, New Zealand

[5] Univ Liege, Fac Vet Med, Immunol Vaccinol, B-4000 Liege, Belgium

来源：

FISH & SHELLFISH IMMUNOLOGY | 2016年 / 57卷

基金：

欧盟第七框架计划;

关键词：

Zebrafish; IFN-gamma; Monoclonal antibody; Protein expression; PHA; Antigen re-stimulation; TROUT ONCORHYNCHUS-MYKISS; SALMON SALMO-SALAR; GINBUNA CRUCIAN CARP; IFN-GAMMA; MOLECULAR CHARACTERIZATION; TAKIFUGU-RUBRIPES; IMMUNE-RESPONSES; GENE-EXPRESSION; T-BET; IDENTIFICATION;

D O I：

10.1016/j.fsi.2016.08.023

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

IFN-gamma is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-gamma protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfiFN-gamma. We show that the zfIFN-gamma moAb specifically recognises E. coli produced recombinant IFN-gamma protein and zfIFN-gamma produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the, production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-gamma protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：79 / 86

页数：8

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