The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism

被引：28
作者：
Francisco Munoz-Valle, Jose [1 ]
Magdalena Torres-Carrillo, Nora [1 ]
Paola Guzman-Guzman, Iris [1 ]
Torres-Carrillo, Norma [1 ]
Luz Ruiz-Quezada, Sandra [2 ]
Azucena Palafox-Sanchez, Claudia [1 ]
Rangel-Villalobos, Hector [3 ]
Guadalupe Ramirez-Duenas, Maria [4 ]
Parra-Rojas, Isela [5 ]
Fafutis-Morris, Mary [4 ]
Estela Bastidas-Ramirez, Blanca [6 ]
Laura Pereira-Suarez, Ana [4 ]
机构：
[1] Univ Guadalajara, Dept Biol Mol & Genom, Ctr Univ Ciencias Salud, Zapopan 45178, Jalisco, Mexico
[2] Univ Guadalajara, Dept Farmacobiol, Ctr Univ Ciencias Exactas & Ingn, Guadalajara 44430, Jalisco, Mexico
[3] Univ Guadalajara, Inst Invest Genet Mol, Ctr Univ Cienega, Ocotlan, Jalisco, Mexico
[4] Univ Guadalajara, Dept Fisiol, Ctr Univ Ciencias Salud, Guadalajara 44430, Jalisco, Mexico
[5] Univ Autonoma Guerrero, Unidad Acad Ciencias Quim Biol, Chilpancingo, Guerrero, Mexico
[6] Univ Guadalajara, Ctr Univ Ciencias Salud, Inst Enfermedades Cron Degenerat, Guadalajara 44430, Jalisco, Mexico
来源：
RHEUMATOLOGY INTERNATIONAL | 2012年 / 32卷 / 02期
关键词：
Rheumatoid arthritis; Polymorphism; TGF-beta; 1; Functional class; TRANSFORMING-GROWTH-FACTOR-BETA-1; GENE; REVISED CRITERIA; JOINT DAMAGE; CLASSIFICATION;
D O I：
10.1007/s00296-010-1624-x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The influence of genetic factors in rheumatoid arthritis (RA) has been described, including several cytokine genes such as transforming growth factor beta (TGF-beta) with regulatory effects on lymphocytes, dendritic cells, macrophages, chondrocytes, and osteoblasts, which are important in the RA pathogenesis. The G915C TGF-beta 1 polymorphism has been associated with soluble TGF-beta 1 (sTGF-beta) serum levels. Thus, we studied the association of G915C (Arg25Pro) TGF-beta 1 polymorphism with sTGF-beta 1 serum levels in RA. We enrolled 120 RA patients and 120 control subjects (CS). The G915C TGF-beta 1 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and sTGF-beta 1 serum levels were quantified using an ELISA kit. The genotype frequency of G915C TGF-beta 1 polymorphism in RA and CS was G/G (91.7%), G/C (8.3%), C/C (0%) and G/G (85.8%), G/C (14.2%), C/C (0%), respectively, without significant differences. Moreover, the G/G TGF-beta 1 genotype carriers presented the highest disability index evaluated for the Spanish HAQ-DI score (P < 0.001). In addition, the sTGF-beta 1 serum levels were higher in RA (182.2 ng/mL) than CS (160.2 ng/mL), there was not significant difference. However, we found a positive correlation between the sTGF-beta 1 serum levels and the functional class (r = 0.472, P = 0.023). In conclusion, the G915C (Arg25Pro) TGF-beta 1 polymorphism is not associated with RA, but the sTGF-beta 1 serum levels are related with the functional class in RA.
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收藏
页码：367 / 372
页数：6
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