Chloroplasts Divide by Contraction of a Bundle of Nanofilaments Consisting of Polyglucan

被引：66

作者：

Yoshida, Yamato ^{[1
]}

Kuroiwa, Haruko ^{[1
]}

Misumi, Osami ^{[1
]}

Yoshida, Masaki ^{[1
]}

Ohnuma, Mio ^{[1
]}

Fujiwara, Takayuki ^{[1
]}

Yagisawa, Fumi ^{[1
]}

Hirooka, Shunsuke ^{[2
]}

Imoto, Yuuta ^{[1
]}

Matsushita, Kazunobu ^{[3
]}

Kawano, Shigeyuki ^{[4
]}

Kuroiwa, Tsuneyoshi ^{[1
]}

机构：

[1] Rikkyo Univ, Cell Biol Lab, Dept Life Sci, Coll Sci,Res Informat Ctr Extremophile, Tokyo 1718501, Japan

[2] Chiba Univ, Dept Nanobiol, Grad Sch Adv Integrat Sci, Chiba 2718510, Japan

[3] Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 7538515, Japan

[4] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Chiba 2778562, Japan

来源：

SCIENCE | 2010年 / 329卷 / 5994期

关键词：

ALGA CYANIDIOSCHYZON-MEROLAE; PLASTID DIVISION; DYNAMIN; MACHINERY; MITOCHONDRIA; GLYCOGENIN; ORGANELLES; APPARATUS; MECHANISM; SEQUENCE;

D O I：

10.1126/science.1190791

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In chloroplast division, the plastid-dividing (PD) ring is a main structure of the PD machinery and is a universal structure in the plant kingdom. However, the components and formation of the PD ring have been enigmatic. By proteomic analysis of PD machineries isolated from Cyanidioschyzon merolae, we identified the glycosyltransferase protein plastid-dividing ring 1 (PDR1), which constructs the PD ring and is widely conserved from red alga to land plants. Electron microscopy showed that the PDR1 protein forms a ring with carbohydrates at the chloroplast-division site. Fluorometric saccharide ingredient analysis of purified PD ring filaments showed that only glucose was included, and down-regulation of PDR1 impaired chloroplast division. Thus, the chloroplasts are divided by the PD ring, which is a bundle of PDR1-mediated polyglucan filaments.

引用

页码：949 / 953

页数：5

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