The effect of heat shock and timing on the induction of tetraploidy in Catfish, Heteropnestus fossilis

被引：2

作者：

Christopher, J. G. ^{[1
]}

Siva, R. ^{[1
]}

Ezilrani, P. ^{[2
]}

机构：

[1] VIT Univ, Sch Bio Sci & Technol, Vellore, Tamil Nadu, India

[2] Cape Breton Univ, Verschuren Ctr, Sydney, NS, Canada

来源：

JOURNAL OF APPLIED AQUACULTURE | 2020年 / 32卷 / 02期

关键词：

Tetraploid; ploidy; heat shock; mitotic division; Heteropnestus fossilis; STINGING CATFISH; VIABLE GYNOGENS; RAINBOW-TROUT; LOACH; SURVIVAL; PLOIDY; CARP; CHROMOSOMES; TRIPLOIDS; PROGENY;

D O I：

10.1080/10454438.2019.1707144

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

This research aims to discover the right physiological age of fertilized egg for tetraploid induction and the parameters of heat shock treatment in Heteropnestus fossilis. The heat shock parameters are shock temperature (39 degrees C, 40 degrees C, and 41 degrees C); time at which the shock is initiated (23 to 28 min after fertilization) and for a duration of 1, 2 and 3 min. The confirmations of tetraploid were based on chromosome counting. The result indicates that the window time to start the heat shock treatment for maximum tetraploid induction is 25 to 27 min after fertilization at an ambient water temperature of 27 degrees C. Shock treatment at 40 degrees C and for 2-min duration, stated at 27 min after fertilization has shown highest tetraploid induction, i.e. 28% (p < .05) with 76% survival. Tetraploids form the main source for the induction of higher ploidy levels.

引用

页码：186 / 192

页数：7

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